非模式物种组织培养和转化研究进展：西番莲属（西番莲科）。

Advances in Tissue Culture and Transformation Studies in Non-model Species: Passiflora spp. (Passifloraceae).

机构信息

Laboratório de Cultura de Tecidos-LCT/BIOAGRO, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil.

Department of Biological Sciences, Center for Biotechnology and Life Sciences, The University of Rhode Island, Kingston, RI, USA.

出版信息

Methods Mol Biol. 2024;2827:207-222. doi: 10.1007/978-1-0716-3954-2_15.

DOI:10.1007/978-1-0716-3954-2_15

PMID:38985273

Abstract

In this chapter, we report advances in tissue culture applied to Passiflora. We present reproducible protocols for somatic embryogenesis, endosperm-derived triploid production, and genetic transformation for such species knowledge generated by our research team and collaborators in the last 20 years. Our research group has pioneered the work on passion fruit somatic embryogenesis, and we directed efforts to characterize several aspects of this morphogenic pathway. Furthermore, we expanded the possibilities of understanding the molecular mechanism related to developmental phase transitions of Passiflora edulis Sims. and P. cincinnata Mast., and a transformation protocol is presented for the overexpression of microRNA156.

摘要

在本章中，我们报告了应用于西番莲属的组织培养进展。我们提供了体细胞胚胎发生、胚乳衍生三倍体生产和遗传转化的可重复方案，这些知识是我们的研究团队和过去 20 年来的合作者生成的。我们的研究小组率先开展了西番莲体细胞胚胎发生的工作，并致力于描述该形态发生途径的几个方面。此外，我们扩大了对与 Passiflora edulis Sims. 和 P. cincinnata Mast. 发育阶段转变相关的分子机制的理解可能性，并提出了用于过表达 microRNA156 的转化方案。