基于离心管胶囊的原位 RT-PCR 新方法，非常适合植物组织中转录本的检测。
A Novel Method for In Situ RT-PCR Based on Capsules from Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues.
作者信息
Laines-Hidalgo José Ignacio, Kú-González Angela F, Vázquez-Flota Felipe A
机构信息
Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Merida, Yucatán, Mexico.
出版信息
Methods Mol Biol. 2024;2827:417-433. doi: 10.1007/978-1-0716-3954-2_28.
In situ RT-PCR presents advantages over other expression analysis methods due to its rapid processing and low-cost equipment. However, this technique is not without its challenges. A protocol based on a capsule made from centrifuge tubes that offers advantages over slides is presented. This capsule protects histological sections from drying out, and its easy assembly reduces time pauses between incubations. In addition, the container size where the sample is deposited allows the addition and withdrawal of the different solutions. The capsule does not need previous sealing after each incubation, and, above all, it is a low-cost and accessible material. A guideline for tissue sectioning using a cryostat that offers advantages over other sectioning methods is also described.
原位 RT-PCR 因其快速处理和低成本设备而优于其他表达分析方法。然而，该技术并非没有挑战。本文提出了一种基于离心管制成的胶囊的方案，该方案优于载玻片。该胶囊可防止组织切片变干，其易于组装可减少孵育之间的时间停顿。此外，用于放置样品的容器尺寸允许添加和去除不同的溶液。胶囊在每次孵育后不需要单独密封，最重要的是，它是一种低成本且易于获取的材料。本文还描述了一种使用冷冻切片机进行组织切片的指南，该方法优于其他切片方法。
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