National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316004, P. R. China.
Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Taizhou, Zhejiang, 318000, P. R. China.
Parasitol Res. 2024 Jul 10;123(7):265. doi: 10.1007/s00436-024-08287-x.
Perkinsus, a parasitic pathogen of marine bivalves, is widely distributed among various mollusks in numerous countries. However, the prevalence and diversity of Perkinsus species in the two economically important mussels, Mytilus coruscus and M. galloprovincialis, in China remain unknown. The presence of the Perkinsus species was identified in the two mussels sampled along the coast of the East China Sea and the Yellow Sea, using both the alternative Ray's fluid thioglycolate medium (ARFTM) and conventional polymerase chain reaction (PCR). The ARFTM test indicated the presence of Perkinsus-like hypnospores in the two mussels. The diameter of the hypnospores in M. coruscus was significantly smaller than that in M. galloprovincialis. The prevalence of Perkinsus in M. galloprovincialis and M. coruscus ranged from 0 to 37.5% and 0 to 25%, respectively. The mean intensity of Perkinsus in M. galloprovincialis and M. coruscus ranged from 0 to 5.14 and 0 to 4.92, respectively. The PCR assay showed that the prevalence of Perkinsus spp. in M. galloprovincialis and M. coruscus was 0 to 25.0% and 0 to 12.5%, respectively. The homology analysis of the newly obtained internal transcribed spacer (ITS) sequences of Perkinsus revealed the highest identity of 100% with P. beihaiensis. The phylogenetic analysis indicated that the Perkinsus isolates from the two mussels were clustered with P. beihaiensis. The results of the molecular biology indicated that only P. beihaiensis was detected in the two mussels. The highest prevalence of P. beihaiensis was observed in Liaoning province (Dalian, 20.83%), followed by Shandong province, Zhejiang province and Fujian province. Consequently, it is recommended that surveillance should be conducted in Dalian, where the prevalence and mean intensity of P. beihaiensis in M. galloprovincialis are the highest.
Perkinsus 是一种寄生在海洋双壳贝类中的病原体，广泛分布于许多国家的各种软体动物中。然而，在中国两种经济上重要的贻贝——贻贝和贻贝中， Perkinsus 种的流行率和多样性尚不清楚。使用替代 Ray's 液体硫代乙醇酸盐培养基 (ARFTM) 和常规聚合酶链反应 (PCR)，在东海和黄海沿岸采集的两种贻贝中均鉴定出 Perkinsus 种的存在。ARFTM 试验表明，两种贻贝中均存在 Perkinsus 样休眠孢子。贻贝中的休眠孢子直径明显小于贻贝。贻贝和贻贝中 Perkinsus 的流行率分别为 0 至 37.5%和 0 至 25%。贻贝和贻贝中 Perkinsus 的平均强度分别为 0 至 5.14 和 0 至 4.92。PCR 检测显示，贻贝和贻贝中 Perkinsus spp.的流行率分别为 0 至 25.0%和 0 至 12.5%。对新获得的 Perkinsus 内转录间隔区 (ITS) 序列的同源性分析显示，与 P. beihaiensis 的同源性最高为 100%。系统发育分析表明，两种贻贝中的 Perkinsus 分离株与 P. beihaiensis 聚类。分子生物学结果表明，仅在两种贻贝中检测到 P. beihaiensis。P. beihaiensis 的最高流行率见于辽宁省（大连，20.83%），其次是山东省、浙江省和福建省。因此，建议在大连进行监测，那里的 P. beihaiensis 在贻贝中的流行率和平均强度最高。
