Laboratory of Fish Diseases, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Laboratory of Fish Diseases, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo 113-8657, Japan.
J Invertebr Pathol. 2019 Sep;166:107226. doi: 10.1016/j.jip.2019.107226. Epub 2019 Jul 29.
During analyses of the invasive Mediterranean mussel Mytilus galloprovincialis for pathologies in Tokyo Bay, infection by the protozoan parasite Perkinsus beihaiensis was found through histological examination, Ray's Fluid Thioglycollate Medium assays, and molecular analyses. Specific PCR assays for each Perkinsus species also revealed the presence of an indigenous congeneric species, Perkinsus olseni, but P. beihaiensis was dominant in M. galloprovincialis. Sequences of the ribosomal internal transcribed spacer region I of P. beihaiensis found in Japan were genetically more similar to those found in South American countries (Panama and Brazil) than in Asian countries (China and India). Though Mediterranean mussels have become widespread in Japanese waters since their invasion in the 1930s, epidemiological surveys show that mussels collected outside Tokyo Bay are free of any Perkinsus infections. Based on these results, it was strongly suggested that P. beihaiensis invaded Tokyo Bay by transportation of bivalves originating from South America but has not yet spread to other parts of Japan. The possibility is not ruled out, however, that the parasite is indigenous in Japan but the environment in Tokyo Bay favors its transmission to Mediterranean mussels.
在对东京湾入侵的地中海贻贝 Mytilus galloprovincialis 进行病理学分析时，通过组织学检查、Ray's 液体硫代糖琼脂培养基检测和分子分析发现了原生动物寄生虫北海派琴虫的感染。针对每种 Perkinsus 物种的特定 PCR 检测也揭示了同种的本地物种 Perkinsus olseni 的存在，但 P. beihaiensis 在 M. galloprovincialis 中占优势。在日本发现的 P. beihaiensis 的核糖体内部转录间隔区 I 序列在遗传上与南美洲国家（巴拿马和巴西）的序列更为相似，而与亚洲国家（中国和印度）的序列则不相似。尽管自 20 世纪 30 年代入侵以来，地中海贻贝已在日本水域广泛分布，但流行病学调查显示，在东京湾以外采集的贻贝没有任何派琴虫感染。基于这些结果，强烈表明 P. beihaiensis 是通过从南美洲运输的双壳类动物传入东京湾的，但尚未传播到日本其他地区。然而，不能排除寄生虫在日本是本地的，但东京湾的环境有利于其传播给地中海贻贝的可能性。
