三种药用天芥菜科植物的完整质体基因组：比较分析与系统发育关系。

Complete plastome genomes of three medicinal heliotropiaceae species: comparative analyses and phylogenetic relationships.

机构信息

Department of Biology, College of Sciences, King Khalid University, Abha, Saudi Arabia.

Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

出版信息

BMC Plant Biol. 2024 Jul 10;24(1):654. doi: 10.1186/s12870-024-05388-8.

DOI:10.1186/s12870-024-05388-8

PMID:38987665

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11234707/

Abstract

BACKGROUND

Heliotropiaceae is a family of the order Boraginales and has over 450 species. The members of the family Heliotropiaceae have been widely reported to be used in traditional medicine Over time, the classification of Heliotropiaceae has remained uncertain and has moved from family to subfamily, or conversely.

RESULTS

In the present study, we sequenced, analyzed, and compared the complete plastomes of Euploca strigosa, Heliotropium arbainense, and Heliotropium longiflorum with the genomes of related taxa. The lengths of the plastomes of E. strigosa, H. arbainense, and H. longiflorum were 155,174 bp, 154,709 bp, and 154,496 bp, respectively. Each plastome consisted of 114 genes: 80 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. The long repeats analysis indicated that reverse, palindromic, complement and forward repeats were all found in the three plastomes. The simple repeats analysis showed that the plastomes of E. strigosa, H. arbainense, and H. longiflorum contained 158, 165, and 151 microsatellites, respectively. The phylogenetic analysis confirmed two major clades in the Boraginales: clade I comprised Boraginaceae, while clade II included Heliotropiaceae, Ehretiaceae, Lennoaceae, and Cordiaceae. Inside the family Heliotropiaceae, E. strigosa is nested within the Heliotropium genus.

CONCLUSIONS

This study expands our knowledge of the evolutionary relationships within Heliotropiaceae and offers useful genetic resources.

摘要

背景

半日花科是茄目茄科的一个科，有超过 450 种。该科的成员已被广泛报道用于传统医学。随着时间的推移，半日花科的分类一直不确定，从科到亚科，或者反之。

结果

在本研究中，我们对 Euploca strigosa、Heliotropium arbainense 和 Heliotropium longiflorum 的完整质体进行了测序、分析和比较，并与相关分类群的基因组进行了比较。E. strigosa、H. arbainense 和 H. longiflorum 的质体长度分别为 155,174 bp、154,709 bp 和 154,496 bp。每个质体由 114 个基因组成：80 个蛋白质编码基因、4 个核糖体 RNA 基因和 30 个转移 RNA 基因。长重复分析表明，反转、回文、互补和正向重复在这三个质体中都有发现。简单重复分析表明，E. strigosa、H. arbainense 和 H. longiflorum 的质体分别含有 158、165 和 151 个微卫星。系统发育分析证实了 Boraginales 中的两个主要分支：分支 I 包括 Boraginaceae，而分支 II 包括半日花科、Ehretiaceae、Lennoaceae 和 Cordiaceae。在半日花科内，E. strigosa 嵌套在半日花属内。