van Meel F C, Pearson P L
Histochemistry. 1979 Oct;63(3):329-39. doi: 10.1007/BF00490061.
Rabbit antisera, specific for the histones F1, F2a2, F2b, F3 and for protamine were used to monitor a possible transition from protamine towards somatic-type histones during sperm nucleus reactivation, following human sperm fusion with mouse fibroblasts. Mature human sperm nuclei were shown to contain the histones F2a2, F2b, F3 and protamine, but were missing F1 histone by immuno cytochemistry using the indirect fluorescence method. However, a gradual disappearance of protamine from fused sperm nuclei, could be observed during the first 24 h of reactivation. Subsequently, F1 histone could be detected in increasing concentrations in 60% of reactivated sperm nuclei, during the next four days. The shift from protamine towards F1 histone could also be visualized cytochemically via staining with brilliant sulphaflavine, which appears to discriminate between sperm nuclei on the basis of their F1 histone content.
使用针对组蛋白F1、F2a2、F2b、F3以及鱼精蛋白的兔抗血清，来监测人精子与小鼠成纤维细胞融合后精子核重新激活过程中从鱼精蛋白向体细胞型组蛋白可能的转变。通过间接荧光法免疫细胞化学显示，成熟的人精子核含有组蛋白F2a2、F2b、F3和鱼精蛋白，但缺少F1组蛋白。然而，在重新激活的最初24小时内，可以观察到融合精子核中鱼精蛋白逐渐消失。随后，在接下来的四天里，在60%重新激活的精子核中可以检测到F1组蛋白的浓度不断增加。从鱼精蛋白向F1组蛋白的转变也可以通过用亮黄磺荧光素染色进行细胞化学观察，亮黄磺荧光素似乎根据精子核的F1组蛋白含量来区分精子核。
