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树脂包埋切片中的免疫过氧化物酶反应。

Immunoperoxidase reactions in resin embedded sections.

作者信息

Heyderman E, Monaghan P

出版信息

Invest Cell Pathol. 1979 Apr-Jun;2(2):119-22.

PMID:389896
Abstract

A simple reproducible and reliable schedule for the immunoperoxidase demonstration of various substances in resin embedded sections is described. It has been applied to a variety of epithelial membrane antigens in carcinoma of the colon and to blood group substances. Preliminary work indicates that it may be applicable to most if not all of the antigens which survive conventional paraffin wax embedding.

摘要

本文描述了一种简单、可重复且可靠的免疫过氧化物酶染色方法,用于在树脂包埋切片中显示各种物质。该方法已应用于结肠癌中多种上皮膜抗原以及血型物质的检测。初步研究表明,它可能适用于大多数(即便不是全部)能在传统石蜡包埋过程中存活下来的抗原。

相似文献

1
Immunoperoxidase reactions in resin embedded sections.树脂包埋切片中的免疫过氧化物酶反应。
Invest Cell Pathol. 1979 Apr-Jun;2(2):119-22.
2
Expression of Lewisa, Lewisb, X, and Y blood group antigens in human colonic tumors and normal tissue and in human tumor-derived cell lines.Lewis a、Lewis b、X和Y血型抗原在人结肠肿瘤、正常组织及人肿瘤衍生细胞系中的表达。
Cancer Res. 1986 Mar;46(3):1553-61.
3
Monoclonal antibody recognizing a carcinoembryonic antigen epitope differentially expressed in human colonic carcinoma versus normal adult colon tissues.识别在人类结肠癌与正常成人结肠组织中差异表达的癌胚抗原表位的单克隆抗体。
Cancer Res. 1989 Oct 15;49(20):5766-73.
4
Sequential demonstration of antigens and AgNORs in frozen and paraffin sections.在冷冻切片和石蜡切片中依次显示抗原和核仁组成区嗜银蛋白
J Pathol. 1989 Oct;159(2):169-72. doi: 10.1002/path.1711590212.
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Immunoperoxidase localization of colonic mucoprotein antigen in neoplastic tissues.
Cancer Res. 1981 Mar;41(3):767-72.
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Evaluation of procedures for the fixation and processing of human tissue for immunohistochemical analysis of human monoclonal antibodies.
Hum Antibodies Hybridomas. 1991 Jul;2(3):135-41.
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Immunochemical characterization of two antigens recognized by new monoclonal antibodies against human colon carcinoma.两种新的抗人结肠癌单克隆抗体识别的抗原的免疫化学特性分析
J Immunol. 1986 Feb 15;136(4):1497-503.
8
Non-co-ordinate expression of HLA-DR antigens and invariant chain.HLA-DR抗原与恒定链的非协调性表达。
Immunology. 1988 Mar;63(3):551-3.
9
A method to generate antigen-specific mAb capable of staining formalin-fixed, paraffin-embedded tissue sections.一种生成能够对福尔马林固定、石蜡包埋组织切片进行染色的抗原特异性单克隆抗体的方法。
J Immunol Methods. 2005 Apr;299(1-2):139-51. doi: 10.1016/j.jim.2005.02.006. Epub 2005 Apr 1.
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Gastrointestinal cancer-associated antigen in immunoperoxidase assay.免疫过氧化物酶测定中的胃肠道癌相关抗原
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引用本文的文献

1
Demonstration of immunoproteins in Araldite-embedded tissues.在环氧树脂包埋组织中免疫蛋白的显示
J Clin Pathol. 1982 Aug;35(8):904. doi: 10.1136/jcp.35.8.904-a.
2
Immunoperoxidase localisation of human placental lactogen: a marker for the placental origin of the giant cells in 'syncytial endometritis' of pregnancy.人胎盘催乳素的免疫过氧化物酶定位:妊娠“合体细胞性子宫内膜炎”中巨细胞胎盘起源的标志物。
J Clin Pathol. 1981 Mar;34(3):303-7. doi: 10.1136/jcp.34.3.303.
3
Alkaline phosphatase for immunocytochemical labelling: problems with endogenous enzyme activity.
用于免疫细胞化学标记的碱性磷酸酶:内源性酶活性问题
J Clin Pathol. 1981 Dec;34(12):1349-51. doi: 10.1136/jcp.34.12.1349.
4
New instrument for longitudinal hemisectioning of undecalcified marrow cores, for simultaneous processing and examination with useful and complimentary techniques.用于未脱钙骨髓芯纵向半切的新型仪器,可同时采用实用且互补的技术进行处理和检查。
J Clin Pathol. 1987 Jan;40(1):112-5. doi: 10.1136/jcp.40.1.112.
5
Immunoperoxidase technique in histopathology: applications, methods, and controls.组织病理学中的免疫过氧化物酶技术:应用、方法与对照
J Clin Pathol. 1979 Oct;32(10):971-8. doi: 10.1136/jcp.32.10.971.