用于免疫细胞化学标记的碱性磷酸酶：内源性酶活性问题
Alkaline phosphatase for immunocytochemical labelling: problems with endogenous enzyme activity.
作者信息
Bulman A S, Heyderman E
出版信息
J Clin Pathol. 1981 Dec;34(12):1349-51. doi: 10.1136/jcp.34.12.1349.
Abstract
Alkaline phosphatase may be used as a label for immunocytochemistry and can be demonstrated in tissue sections using the single step naphthol phosphate method. Endogenous enzyme activity may not be destroyed by fixation in formalin, formol alcohol, Carnoy's or Baker's solutions and should be inhibited before results are assessed. Either Bouin's solution or periodic acid followed by potassium borohydride are satisfactory inhibitor and do not adversely affect immunocytochemical results.
摘要
碱性磷酸酶可用作免疫细胞化学的标记物，并且可以使用单步萘酚磷酸法在组织切片中显示出来。内源性酶活性不会因在福尔马林、甲醛酒精、卡诺氏液或贝克氏液中固定而被破坏，在评估结果之前应予以抑制。布因氏液或高碘酸继以硼氢化钾都是令人满意的抑制剂，且不会对免疫细胞化学结果产生不利影响。
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本文引用的文献
1
Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.
Immunochemistry. 1969 Jan;6(1):43-52. doi: 10.1016/0019-2791(69)90177-3.
2
Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.
J Histochem Cytochem. 1974 Jan;22(1):51-4. doi: 10.1177/22.1.51.
3
Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.
J Histochem Cytochem. 1972 Oct;20(10):829-31. doi: 10.1177/20.10.829.
4
Enumeration of lymphocyte populations in whole peripheral blood with alkaline phosphatase-labelled reagents. A method for routine clinical use.
Clin Exp Immunol. 1977 Jul;29(1):162-7.
5
Immunoperoxidase reactions in resin embedded sections.
Invest Cell Pathol. 1979 Apr-Jun;2(2):119-22.
6
Immunoperoxidase technique in histopathology: applications, methods, and controls.
J Clin Pathol. 1979 Oct;32(10):971-8. doi: 10.1136/jcp.32.10.971.
7
Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute.
Am J Clin Pathol. 1979 May;71(5):483-8. doi: 10.1093/ajcp/71.5.483.
8
Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.
J Clin Pathol. 1978 May;31(5):454-60. doi: 10.1136/jcp.31.5.454.
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