Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi 835215, India.
Centre for Health Care Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur 711103, India.
J Pharm Pharmacol. 2024 Sep 3;76(9):1225-1235. doi: 10.1093/jpp/rgae088.
Patients with type 2 diabetes or prolonged diabetic condition are webbed into cardiac complications. This study aimed to ascertain the utility of chick embryo as an alternative to the mammalian model for type 2 diabetes-induced cardiac complications and chrysin as a protective agent.
Diabetes was activated in ovo model (chick embryo) using glucose along with β-hydroxybutyric acid. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Alamar, and Kenacid blue assay were used to compare with chrysin-administered group. Blood glucose level, total cholesterol, triglyceride, and high-density lipoprotein were considered as endpoints. Diabetes was induced in Wistar albino rats by administering a high-fat diet and a subdued dose of streptozotocin (35 mg/kg, b.w). Percentage of glycated hemoglobin, creatinine kinase-MB, tumor necrosis factor-α, and C-reactive protein were evaluated and compared with chrysin administered group.
Chrysin treatment improved elevated blood glucose levels and dyslipidemia in a diabetic group of whole embryos. Condensed cellular growth and protein content as well as enhanced cytotoxicity in ovo were shielded by chrysin. Chrysin reduced cardiac and inflammatory markers in diabetic rats and provided cellular protection to damage the heart of diabetic rats.
The protective action of chrysin in ovo model induced a secondary complication associated with diabetes, evidenced that the ovo model is an effective alternative in curtailing higher animal use in scientific research.
2 型糖尿病或长期糖尿病患者易发生心脏并发症。本研究旨在确定鸡胚是否可替代哺乳动物模型用于 2 型糖尿病诱导的心脏并发症，以及白杨素是否具有保护作用。
在鸡胚（鸡胚胎）中使用葡萄糖和β-羟丁酸激活糖尿病。使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐、阿马尔蓝和肯酸蓝测定法与白杨素给药组进行比较。血糖水平、总胆固醇、甘油三酯和高密度脂蛋白被视为终点。通过给予高脂肪饮食和低剂量链脲佐菌素（35mg/kg，bw）诱导 Wistar 白化大鼠糖尿病。评估并比较糖化血红蛋白、肌酸激酶-MB、肿瘤坏死因子-α和 C 反应蛋白的百分比与白杨素给药组。
白杨素治疗可改善整个胚胎糖尿病组的高血糖水平和血脂异常。白杨素可防止鸡胚中细胞生长和蛋白质含量的凝聚以及细胞毒性的增强。白杨素可降低糖尿病大鼠的心脏和炎症标志物，并为损伤糖尿病大鼠的心脏提供细胞保护。
白杨素在鸡胚模型中诱导的与糖尿病相关的继发性并发症的保护作用表明，该模型是减少科学研究中高动物使用的有效替代方法。
