Schwarz L C, Makowka L, Falk J A, Falk R
Ann Surg. 1985 Sep;202(3):296-302. doi: 10.1097/00000658-198509000-00004.
This report presents further evidence that the liver is the source of the factor responsible for the initiation and/or stimulation of hepatic regeneration. Initial experiments for the isolation and characterization of the active factor are presented. The factor was isolated from the cytosol of regenerating livers (RLC). After an in vivo exposure to RLC, hepatocytes were pulsed in vitro with 3H-thymidine to measure DNA synthesis. Rat and porcine RLC stimulated DNA synthesis in hepatocytes isolated from growing (nonhepatectomized) livers of weanling rats, or from regenerating livers of adult rats. The ability of porcine RLC to stimulate hepatocyte DNA synthesis demonstrated that the factor responsible was not species-specific. In contrast, normal non-regenerating liver cytosol did not stimulate hepatocyte DNA synthesis. Further experiments also revealed that the factor is heat stable. The activity responsible for the increased DNA synthesis was called hepatocyte proliferation factor (HPF). The assay for detecting HPF activity in the nonhepatectomized recipient will facilitate further characterization and purification of HPF.
本报告提供了进一步的证据，表明肝脏是负责启动和/或刺激肝脏再生的因子的来源。文中展示了分离和鉴定该活性因子的初步实验。该因子是从再生肝脏的胞质溶胶（RLC）中分离出来的。在体内暴露于RLC后，用3H-胸腺嘧啶核苷对肝细胞进行体外脉冲处理以测量DNA合成。大鼠和猪的RLC刺激了从断奶大鼠生长中的（未切除肝脏的）肝脏或成年大鼠再生肝脏中分离出的肝细胞的DNA合成。猪RLC刺激肝细胞DNA合成的能力表明，所涉及的因子并非物种特异性的。相比之下，正常的非再生肝脏胞质溶胶不会刺激肝细胞DNA合成。进一步的实验还表明该因子具有热稳定性。负责增加DNA合成的活性被称为肝细胞增殖因子（HPF）。在未切除肝脏的受体中检测HPF活性的测定方法将有助于对HPF进行进一步的鉴定和纯化。
