Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi, Hyderabad 502284, India.
G Pullareddy College of Pharmacy, Mehadipatnam, Hyderabad 500028, India.
ACS Infect Dis. 2024 Aug 9;10(8):2978-2990. doi: 10.1021/acsinfecdis.4c00306. Epub 2024 Jul 11.
Infectious diabetic wounds present a substantial challenge, characterized by inflammation, infection, and delayed wound healing, leading to elevated morbidity and mortality rates. In this work, we developed a multifunctional lipid nanoemulsion containing quercetin, chlorine e6, and rosemary oil (QCRLNEs) for dual anti-inflammatory and antibacterial photodynamic therapy (APDT) for treating infectious diabetic wounds. The QCRLNEs exhibited spherical morphology with a size of 51 nm with enhanced encapsulation efficiency, skin permeation, and localized delivery at the infected wound site. QCRLNEs with NIR irradiation have shown excellent wound closure and antimicrobial properties in vitro, mitigating the nonselective cytotoxic behavior of PDT. Also, excellent biocompatibility and anti-inflammatory and wound healing responses were observed in zebrafish models. The infected wound healing properties in -infected diabetic rat models indicated re-epithelization and collagen deposition with no signs of inflammation. This multifaceted approach using QCRLNEs with NIR irradiation holds great promise for effectively combating oxidative stress and bacterial infections commonly associated with infected diabetic wounds, facilitating enhanced wound healing and improved clinical outcomes.
感染性糖尿病伤口是一个重大挑战，其特征为炎症、感染和伤口愈合延迟，导致发病率和死亡率升高。在这项工作中，我们开发了一种载有槲皮素、氯己定和迷迭香油的多功能脂质纳米乳液（QCRLNEs），用于治疗感染性糖尿病伤口的双重抗炎和抗菌光动力疗法（APDT）。QCRLNEs 呈球形，粒径为 51nm，具有增强的包封效率、皮肤渗透性和在感染伤口部位的局部递送。用近红外光照射的 QCRLNEs 在体外表现出良好的伤口闭合和抗菌性能，减轻了光动力疗法的非选择性细胞毒性行为。此外，在斑马鱼模型中观察到了优异的生物相容性、抗炎和伤口愈合反应。感染性糖尿病大鼠模型中的感染性伤口愈合特性表明，再上皮化和胶原蛋白沉积，没有炎症迹象。这种使用近红外光照射的 QCRLNEs 的多方面方法有望有效对抗与感染性糖尿病伤口相关的氧化应激和细菌感染，促进伤口愈合的增强和临床结果的改善。
