Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nat Commun. 2024 Jul 11;15(1):5833. doi: 10.1038/s41467-024-49461-0.
Arthropod-borne viruses represent a crucial public health threat. Current arboviral serology assays are either labor intensive or incapable of distinguishing closely related viruses, and many zoonotic arboviruses that may transition to humans lack any serologic assays. In this study, we present a programmable phage display platform, ArboScan, that evaluates antibody binding to overlapping peptides that represent the proteomes of 691 human and zoonotic arboviruses. We confirm that ArboScan provides detailed antibody binding information from animal sera, human sera, and an arthropod blood meal. ArboScan identifies distinguishing features of antibody responses based on exposure history in a Colombian cohort of Zika patients. Finally, ArboScan details epitope level information that rapidly identifies candidate epitopes with potential protective significance. ArboScan thus represents a resource for characterizing human and animal arbovirus antibody responses at cohort scale.
虫媒病毒是对公共健康的重大威胁。目前的虫媒病毒血清学检测方法要么劳动强度大，要么无法区分密切相关的病毒，而且许多可能传播给人类的人畜共患虫媒病毒缺乏任何血清学检测方法。在这项研究中，我们提出了一个可编程的噬菌体展示平台，ArboScan，它评估了针对 691 种人类和人畜共患虫媒病毒的蛋白质组的重叠肽的抗体结合情况。我们证实，ArboScan 从动物血清、人类血清和节肢动物血餐中提供了详细的抗体结合信息。ArboScan 根据哥伦比亚寨卡病毒患者队列的暴露史确定了抗体反应的特征。最后，ArboScan 详细描述了表位水平的信息，快速确定了具有潜在保护意义的候选表位。因此，ArboScan 代表了在队列规模上描述人类和动物虫媒病毒抗体反应的资源。
