• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

RsrC-RsrA-RsrB 转录调控回路正向调控草酸青霉多糖降解酶生物合成和发育。

A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum.

机构信息

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, P. R. China.

Guangxi Research Center for Microbial and Enzyme Engineering Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, P. R. China.

出版信息

Commun Biol. 2024 Jul 11;7(1):848. doi: 10.1038/s42003-024-06536-4.

DOI:10.1038/s42003-024-06536-4
PMID:38992164
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11239660/
Abstract

Filamentous fungi produce polysaccharide-degrading enzymes, which is controlled by poorly understood transcriptional circuits. Here we show that a circuit comprising RsrC-RsrA-RsrB (Rsr: production of raw-starch-degrading enzyme regulator) that positively regulates production of raw starch-degrading enzymes in Penicillium oxalicum. Transcription factor (TF) RsrA is essential for biosynthesis of raw starch-degrading enzymes. RsrB and RsrC containing Zn2Cys6- and C2H2-zinc finger domains, act downstream and upstream of RsrA, respectively. RsrA activates rsrB transcription, and three nucleotides (G, G and G) of rsrB promoter region are required for RsrA, in terms of TF, for binding. RsrB binds to DNA sequence 5'-TCGATCAGGCACGCC-3' in the promoter region of the gene encoding key raw-starch-degrading enzyme PoxGA15A. RsrC specifically binds rsrA promoter, but not amylase genes, to positively regulate the expression of rsrA and the production of raw starch-degrading enzymes. These findings expand complex regulatory network of fungal raw starch-degrading enzyme biosynthesis.

摘要

丝状真菌产生多糖降解酶，其受转录调控回路的调控。本文显示，由 RsrC-RsrA-RsrB（Rsr：生成分解 raw-starch 酶的调节因子）组成的回路正向调控草酸青霉中 raw-starch 降解酶的产生。转录因子（TF）RsrA 是 raw-starch 降解酶生物合成所必需的。RsrB 和 RsrC 分别含有 Zn2Cys6 和 C2H2 锌指结构域，位于 RsrA 的下游和上游。RsrA 激活 rsrB 转录，而 rsrB 启动子区域的三个核苷酸（G、G 和 G）是 RsrA 结合的必需条件。RsrB 结合到编码关键 raw-starch 降解酶 PoxGA15A 的基因启动子区域中的 5'-TCGATCAGGCACGCC-3'序列。RsrC 特异性结合 rsrA 启动子，但不结合淀粉酶基因，以正向调节 rsrA 的表达和 raw-starch 降解酶的产生。这些发现扩展了真菌 raw-starch 降解酶生物合成的复杂调控网络。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/951c95fb2c39/42003_2024_6536_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/9a32a7114bcf/42003_2024_6536_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/3afa0dc070ae/42003_2024_6536_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/d6e335d2ec38/42003_2024_6536_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/dfcbf1c3ce00/42003_2024_6536_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/776c1fae74ef/42003_2024_6536_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/4eb9dc156a68/42003_2024_6536_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/1821809c3747/42003_2024_6536_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/8c3481295edc/42003_2024_6536_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/3b9cdbeecc4c/42003_2024_6536_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/951c95fb2c39/42003_2024_6536_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/9a32a7114bcf/42003_2024_6536_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/3afa0dc070ae/42003_2024_6536_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/d6e335d2ec38/42003_2024_6536_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/dfcbf1c3ce00/42003_2024_6536_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/776c1fae74ef/42003_2024_6536_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/4eb9dc156a68/42003_2024_6536_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/1821809c3747/42003_2024_6536_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/8c3481295edc/42003_2024_6536_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/3b9cdbeecc4c/42003_2024_6536_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9fb/11239660/951c95fb2c39/42003_2024_6536_Fig10_HTML.jpg

相似文献

1
A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum.RsrC-RsrA-RsrB 转录调控回路正向调控草酸青霉多糖降解酶生物合成和发育。
Commun Biol. 2024 Jul 11;7(1):848. doi: 10.1038/s42003-024-06536-4.
2
Mutual regulation of novel transcription factors RsrD and RsrE positively modulates the production of raw-starch-degrading enzyme in .新型转录因子 RsrD 和 RsrE 的相互调控正向调节. 中 raw-starch-degrading 酶的产生。
Appl Environ Microbiol. 2024 Aug 21;90(8):e0039024. doi: 10.1128/aem.00390-24. Epub 2024 Jul 18.
3
Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum.转录因子 NsdD 调控草酸青霉中参与植物生物质降解酶、分生孢子形成和色素生物合成的基因表达。
Appl Environ Microbiol. 2018 Aug 31;84(18). doi: 10.1128/AEM.01039-18. Print 2018 Sep 15.
4
Secretory overproduction of a raw starch-degrading glucoamylase in Penicillium oxalicum using strong promoter and signal peptide.利用强启动子和信号肽在草酸青霉中过表达生淀粉降解葡萄糖淀粉酶。
Appl Microbiol Biotechnol. 2018 Nov;102(21):9291-9301. doi: 10.1007/s00253-018-9307-8. Epub 2018 Aug 28.
5
Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum.通过基因工程与随机诱变相结合提高草酸青霉中 raw-starch-degrading enzymes 的产量。
Microb Cell Fact. 2022 Dec 24;21(1):272. doi: 10.1186/s12934-022-01997-w.
6
Regulatory function of the novel transcription factor CxrC in Penicillium oxalicum.新型转录因子 CxrC 在草酸青霉中的调控功能。
Mol Microbiol. 2021 Dec;116(6):1512-1532. doi: 10.1111/mmi.14843. Epub 2021 Nov 30.
7
Kinase POGSK-3β modulates fungal plant polysaccharide-degrading enzyme production and development.激酶 POGSK-3β 调节真菌植物多糖降解酶的产生和发育。
Appl Microbiol Biotechnol. 2023 Jun;107(11):3605-3620. doi: 10.1007/s00253-023-12548-7. Epub 2023 Apr 29.
8
Regulation of fungal raw-starch-degrading enzyme production depends on transcription factor phosphorylation and recruitment of the Mediator complex.真菌源生淀粉降解酶产量的调控取决于转录因子磷酸化和中介体复合物的募集。
Commun Biol. 2023 Oct 12;6(1):1032. doi: 10.1038/s42003-023-05404-x.
9
Penicillium oxalicum PoFlbC regulates fungal asexual development and is important for cellulase gene expression.草酸青霉PoFlbC调节真菌无性发育，对纤维素酶基因表达很重要。
Fungal Genet Biol. 2016 Jan;86:91-102. doi: 10.1016/j.fgb.2015.12.012. Epub 2015 Dec 23.
10
Characterization of novel roles of a HMG-box protein PoxHmbB in biomass-degrading enzyme production by Penicillium oxalicum.新型 HMG 盒蛋白 PoxHmbB 在草酸青霉产生物质降解酶中的新作用特征。
Appl Microbiol Biotechnol. 2018 Apr;102(8):3739-3753. doi: 10.1007/s00253-018-8867-y. Epub 2018 Mar 6.

本文引用的文献

1
Regulation of fungal raw-starch-degrading enzyme production depends on transcription factor phosphorylation and recruitment of the Mediator complex.真菌源生淀粉降解酶产量的调控取决于转录因子磷酸化和中介体复合物的募集。
Commun Biol. 2023 Oct 12;6(1):1032. doi: 10.1038/s42003-023-05404-x.
2
Kinase POGSK-3β modulates fungal plant polysaccharide-degrading enzyme production and development.激酶 POGSK-3β 调节真菌植物多糖降解酶的产生和发育。
Appl Microbiol Biotechnol. 2023 Jun;107(11):3605-3620. doi: 10.1007/s00253-023-12548-7. Epub 2023 Apr 29.
3
Protein Kinase PoxMKK1 Regulates Plant-Polysaccharide-Degrading Enzyme Biosynthesis, Mycelial Growth and Conidiation in .
蛋白激酶PoxMKK1调节植物多糖降解酶的生物合成、菌丝生长及分生孢子形成 。
J Fungi (Basel). 2023 Mar 23;9(4):397. doi: 10.3390/jof9040397.
4
The interaction between the histone acetyltransferase complex Hat1-Hat2 and transcription factor AmyR provides a molecular brake to regulate amylase gene expression.组蛋白乙酰转移酶复合物 Hat1-Hat2 与转录因子 AmyR 的相互作用为调节淀粉酶基因表达提供了分子制动机制。
Mol Microbiol. 2023 Apr;119(4):471-491. doi: 10.1111/mmi.15036. Epub 2023 Feb 14.
5
Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum.通过基因工程与随机诱变相结合提高草酸青霉中 raw-starch-degrading enzymes 的产量。
Microb Cell Fact. 2022 Dec 24;21(1):272. doi: 10.1186/s12934-022-01997-w.
6
Genetic modifications of critical regulators provide new insights into regulation modes of raw-starch-digesting enzyme expression in Penicillium.关键调控因子的基因改造为深入了解青霉中直链淀粉消化酶表达的调控模式提供了新的见解。
Biotechnol Biofuels Bioprod. 2022 May 31;15(1):62. doi: 10.1186/s13068-022-02162-6.
7
Three-Dimensional Genome Map of the Filamentous Fungus .丝状真菌的三维基因组图谱
Microbiol Spectr. 2022 Jun 29;10(3):e0212121. doi: 10.1128/spectrum.02121-21. Epub 2022 May 2.
8
Promoter regulation and genetic engineering strategies for enhanced cellulase expression in Trichoderma reesei.启动子调控和遗传工程策略在里氏木霉中增强纤维素酶表达。
Microbiol Res. 2022 Jun;259:127011. doi: 10.1016/j.micres.2022.127011. Epub 2022 Mar 21.
9
Simultaneous manipulation of transcriptional regulator CxrC and translational elongation factor eEF1A enhances the production of plant-biomass-degrading enzymes of Penicillium oxalicum.同时操纵转录调节因子CxrC和翻译延伸因子eEF1A可提高草酸青霉植物生物质降解酶的产量。
Bioresour Technol. 2022 May;351:127058. doi: 10.1016/j.biortech.2022.127058. Epub 2022 Mar 23.
10
Regulatory function of the novel transcription factor CxrC in Penicillium oxalicum.新型转录因子 CxrC 在草酸青霉中的调控功能。
Mol Microbiol. 2021 Dec;116(6):1512-1532. doi: 10.1111/mmi.14843. Epub 2021 Nov 30.