Department of Periodontology, Faculty of Dentistry, Gazi University, Biskek cad. 1. Sokak No:4, Emek Ankara, 06490, Turkey.
Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Clin Oral Investig. 2024 Jul 12;28(8):426. doi: 10.1007/s00784-024-05794-0.
To assess the short-term efficacy of multiple sessions of antimicrobial photodynamic therapy (aPDT), light-emitting-diode (LED) photobiomodulation, and topical ozone therapy applications following surgical regenerative treatments on clinical parameters, patient-centered outcomes, and mRNA expression levels of VEGF, IL-6, RunX2, Nell-1, and osterix in gingival crevicular fluid samples in patients with stage III/IV, grade C periodontitis.
Forty-eight systemically healthy patients were assigned into four groups to receive adjunctive modalities with regenerative periodontal surgical treatment. A 970 ± 15 nm diode laser plus indocyanine-green for aPDT group, a 626 nm LED for photobiomodulation group, and topical gaseous ozone were applied at 0, 1, 3, and 7 postoperative days and compared to control group. The clinical periodontal parameters, early wound healing index (EHI), and postoperative patients' morbidity were evaluated. The mRNA levels of biomarkers were assessed by real-time polymerase chain reaction.
No significant difference in the clinical parameters except gingival recession (GR) was identified among the groups. For group-by-time interactions, plaque index (PI) and probing pocket depths (PD) showed significant differences (p = 0.034; p = 0.022). In sites with initial PD > 7 mm, significant differences were observed between control and photobiomodulation groups in PD (p = 0.011), between control and aPDT, and control and photobiomodulation groups in CAL at 6-month follow-up (p = 0.007; p = 0.022). The relative osterix mRNA levels showed a statistically significant difference among the treatment groups (p = 0.014).
The additional applications of aPDT and LED after regenerative treatment of stage III/IV grade C periodontitis exhibited a more pronounced beneficial effect on clinical outcomes in deep periodontal pockets.
评估多次应用抗菌光动力疗法（aPDT）、发光二极管（LED）光生物调节和局部臭氧治疗对 III/IV 期、C 级牙周炎患者手术再生治疗后临床参数、以患者为中心的结果以及龈沟液样本中 VEGF、IL-6、RunX2、Nell-1 和骨钙素 mRNA 表达水平的影响。
将 48 名系统性健康患者分为四组，分别接受再生牙周手术治疗的辅助治疗。aPDT 组使用 970±15nm 二极管激光联合吲哚菁绿，光生物调节组使用 626nm LED，局部气态臭氧分别在术后 0、1、3 和 7 天应用，并与对照组进行比较。评估临床牙周参数、早期愈合指数（EHI）和术后患者发病率。通过实时聚合酶链反应评估生物标志物的 mRNA 水平。
除牙龈退缩（GR）外，各组间临床参数无显著差异。对于组间时间交互作用，菌斑指数（PI）和探诊袋深度（PD）显示出显著差异（p=0.034;p=0.022）。在初始 PD>7mm 的部位，与对照组和光生物调节组相比，PD 存在显著差异（p=0.011），与对照组和 aPDT 组以及对照组和光生物调节组相比，CAL 在 6 个月随访时存在显著差异（p=0.007;p=0.022）。治疗组之间骨钙素 mRNA 水平存在统计学差异（p=0.014）。
在 III/IV 期 C 级牙周炎的再生治疗后，额外应用 aPDT 和 LED 对深牙周袋的临床结果具有更显著的有益作用。
