Poleti Marcelo Lupion, Gregório Danielle, Bistaffa Alisson Gabriel Idelfonso, Vilhena Fabiano Vieira, Simão Andréa Name Colado, Mori Mayara Tiemi Enokida, Stadtlober Nicole Perugini, Lozovoy Marcell Alysson Batisti, da Silva Santos Paulo Sérgio, Tatibana Berenice Tomoko, Fernandes Thais Maria Freire
Oral Health, Federal Institute of Paraná - IFPR, Londrina, Brazil.
Postgraduate Program in Dentistry, School of Dentistry, University of North Paraná - UNOPAR/UNIDERP, Londrina, Brazil.
GMS Hyg Infect Control. 2024 Jun 5;19:Doc32. doi: 10.3205/dgkh000487. eCollection 2024.
The aim of this study was to assess whether toothbrushing with a dentifrice containing an antimicrobial phthalocyanine derivative (APD) can reduce the intraoral viral load of SARS-CoV-2. Twenty COVID-19-positive dentate patients aged ≥18 years were selected instructed to brush their teeth for 2 min with a dentifrice containing APD. Self-collected samples of unstimulated saliva were carried out three times: T0 (baseline), T5 (5 min after toothbrushing), and T30 (30 min after toothbrushing). The analysis of viral RNA was performed by RT-qPCR for detection of three viral genes (ORF1ab, N and S genes). Results were statistically tested using Friedman's test and pairwise comparison with Bonferroni corrections, with a significance level of 5%. There was an increase in the cycle threshold (Ct) value from T0 to T5 in 13 patients (72.2%), and from T0 to T30 in 14 patients (77.8%). In two patients (11.1%) no SARS-CoV-2 was detected at T5 and five patients (27.8%) at T30. The Ct values were statistically significantly higher (p=0.020) at T30 in comparison to T0 and T5. This pilot study suggests that toothbrushing with a dentifrice containing APD could reduce the SARS-CoV-2 viral load in the oral cavity. However, further studies are needed to confirm this possible beneficial effect against SARS-CoV-2.
本研究的目的是评估使用含有抗菌酞菁衍生物（APD）的牙膏刷牙是否可以降低严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的口腔病毒载量。选取了20名年龄≥18岁的COVID-19阳性有牙患者，指导他们使用含有APD的牙膏刷牙2分钟。自行采集未刺激唾液样本3次：T0（基线）、T5（刷牙后5分钟）和T30（刷牙后30分钟）。通过逆转录定量聚合酶链反应（RT-qPCR）对病毒RNA进行分析，以检测三个病毒基因（开放阅读框1ab、N和S基因）。使用弗里德曼检验进行统计测试，并采用邦费罗尼校正进行两两比较，显著性水平为5%。13名患者（72.2%）从T0到T5以及14名患者（77.8%）从T0到T30的循环阈值（Ct）值有所增加。两名患者（11.1%）在T5时未检测到SARS-CoV-2，五名患者（27.8%）在T30时未检测到。与T0和T5相比，T30时的Ct值在统计学上显著更高（p=0.020）。这项初步研究表明，使用含有APD的牙膏刷牙可能会降低口腔中的SARS-CoV-2病毒载量。然而，需要进一步的研究来证实这种对SARS-CoV-2可能的有益作用。
