Jimbow K, Maeda K, Ito Y, Ishida O, Takami T
Cancer. 1985 Nov 15;56(10):2458-69. doi: 10.1002/1097-0142(19851115)56:10<2458::aid-cncr2820561022>3.0.co;2-s.
This study characterized, by means of immunocytochemistry and electron microscopy, four cases of "unusual" cutaneous T-cell lymphoma (CTCL) other than classical mycosis fungoids and Sézary syndrome. Cases 1, 2, and 4 were diffuse lymphoma of a pleomorphic type, and Case 3 was of a mixed type. Case 4 shared a feature common to pagetoid reticulosis. A fairly large number of inflammatory cells were seen in Cases 1, 3, and 4. Functionally, the neoplastic cells of Cases 1, 3, and 4 were of a helper/inducer T-cell subset, whereas those of Case 2 were of a suppressor/cytotoxic T-cell type. Epidermotropic cells with pagetoid growth in Case 4 failed to show these specific surface phenotypes, although they still retained pan T-cell markers. Neoplastic large or intermediate-sized cells revealed a marked difference in the development of cytoplasmic organelles and their nuclear profiles, ranging from a few simple indentations (Cases 2 and 3) to forms with many deep indentations (Case 1) and highly cleaved shapes (Case 4). All of these cells, however, possessed dense-cored granules located in a portion of the cytoplasm. This study indicated the clinicopathologic and immunologic heterogeneity of CTCL, which may be classified, according to the reactivity with monoclonal antibodies and the fine structural features, into subtypes that correspond to functionally distinct subsets of T-cells and their stages or types of differentiation.
本研究通过免疫细胞化学和电子显微镜对4例除经典蕈样肉芽肿和 Sézary 综合征之外的“不寻常”皮肤T细胞淋巴瘤（CTCL）进行了特征描述。病例1、2和4为多形性弥漫性淋巴瘤，病例3为混合型。病例4具有派杰样网状细胞增生症的共同特征。在病例1、3和4中可见相当数量的炎症细胞。从功能上看，病例1、3和4的肿瘤细胞属于辅助/诱导性T细胞亚群，而病例2的肿瘤细胞属于抑制/细胞毒性T细胞类型。病例4中具有派杰样生长的亲表皮细胞未显示出这些特异性表面表型，尽管它们仍保留泛T细胞标志物。肿瘤性大或中等大小的细胞在细胞质细胞器发育及其核形态方面显示出明显差异，从一些简单的凹陷（病例2和3）到有许多深凹陷的形态（病例1）和高度裂解的形状（病例4）不等。然而，所有这些细胞在细胞质的一部分中都含有致密核心颗粒。本研究表明CTCL在临床病理和免疫方面具有异质性，根据与单克隆抗体的反应性和精细结构特征，可分为与功能上不同的T细胞亚群及其分化阶段或类型相对应的亚型。
