Nicolas M T, Johnson C H, Bassot J M, Hastings J W
Cell Biol Int Rep. 1985 Sep;9(9):797-802. doi: 10.1016/0309-1651(85)90098-0.
A polyclonal antibody directed against the luciferase of the luminous dinoflagellate Gonyaulax polyedra labels both dense vesicles and trichocyst sheaths, as visualized in the electron microscope after treatment of antibody-reacted sections with an immunogold probe. Because of their similar size, shape and localization, the dense vesicles seen with the electron microscope are postulated to correspond to autofluorescent particles seen with the fluorescent microscope, which are known to be the origin of bioluminescent flashes in this alga. The explanation for the trichocyst sheath-specific labeling is less evident. The possibility that a second antibody of different specificity is involved has not been excluded but seems unlikely. Alternatively, it could be due to a different but antigenically cross-reacting protein. But the possibility that luciferase itself occurs in two different organelles is intriguing and consistent with previous biochemical studies of cell extracts.
一种针对发光甲藻多甲藻（Gonyaulax polyedra）荧光素酶的多克隆抗体标记了致密小泡和刺丝囊鞘，在用免疫金探针处理抗体反应切片后，在电子显微镜下可见。由于电子显微镜下看到的致密小泡大小、形状和定位相似，推测它们与荧光显微镜下看到的自发荧光颗粒相对应，已知这些颗粒是这种藻类生物发光闪光的来源。刺丝囊鞘特异性标记的解释不太明显。尚未排除涉及不同特异性第二抗体的可能性，但似乎不太可能。或者，这可能是由于一种不同但抗原交叉反应的蛋白质。但是荧光素酶本身存在于两种不同细胞器中的可能性很有趣，并且与之前对细胞提取物的生化研究一致。
