Nicolas M T, Sweeney B M, Hastings J W
Department of Biological Sciences, University of California, Santa Barbara 93106.
J Cell Sci. 1987 Feb;87 ( Pt 1):189-96. doi: 10.1242/jcs.87.1.189.
In order to discover the intracellular location of luciferase in dinoflagellates, sections from a number of species were treated with a polyclonal anti-luciferase and the bound antibody was visualized at the electron-microscope level by indirect immunogold labelling. In two species of Pyrocystis and in Noctiluca, as in Gonyaulax, antibody became bound to dense vesicles, which correspond in size and position to light-emitting bodies detected in previous work. These vesicles resemble microsomes, are bounded by a single membrane and sometimes project into the vacuole. Unexpectedly, the trichocysts of Gonyaulax and Noctiluca and the related mucocysts of Pyrocystis also bound the antibody. This cross-reaction seems quite independent of bioluminescence, since the trichocysts of the non-luminous Cachonina also reacted positively. The possibility is discussed that a protein, different from luciferase but having some antigenic similarity, is present in trichocysts and related organelles.
为了确定荧光素酶在甲藻中的细胞内定位，对多个物种的切片用多克隆抗荧光素酶抗体进行处理，并通过间接免疫金标记在电子显微镜水平观察结合的抗体。在两种多甲藻属物种以及夜光藻中，与在多甲藻中一样，抗体与致密囊泡结合，这些囊泡的大小和位置与先前工作中检测到的发光体相对应。这些囊泡类似于微粒体，由单层膜包围，有时会伸入液泡中。出乎意料的是，多甲藻和夜光藻的刺丝囊以及多甲藻相关的黏液囊也能结合抗体。这种交叉反应似乎与生物发光完全无关，因为不发光的卡氏藻的刺丝囊也呈阳性反应。文中讨论了一种可能性，即刺丝囊和相关细胞器中存在一种不同于荧光素酶但具有某些抗原相似性的蛋白质。
