Zhang Li, Wang Jian-Yu, Zhao Cai-Yan, Shen Chuan, Chen Mei-Ru, Tian Zhi-Ying
Department of Gastroenterology, Hengshui People's Hospital, Hengshui 053000, Hebei Province, China.
Department of Neurology, Hengshui People's Hospital, Hengshui 053000, Hebei Province, China.
World J Clin Cases. 2024 Jul 6;12(19):3845-3853. doi: 10.12998/wjcc.v12.i19.3845.
Stromal cell derived factor-1 (SDF-1) plays a pivotal role in the recruitment of stem cells to injured livers. However, the changes of SDF-l in patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) have yet to be elucidated.
To study the SDF-1 changes in patients with HBV-related ACLF.
30 patients with HBV-related ACLF, 27 patients with chronic hepatitis B and 20 healthy individuals are involved in our study. The SDF-l mRNA expression in liver tissue was detected by quantitative real-time polymerase chain reaction. Immunohistochemical staining was performed to illustrate the expression of SDF-l, CXC receptor 4 (CXCR4) and Ki67. The serum SDF-l concentrations were also detected by enzyme-linked immunosorbent assays.
The expression of SDF-1 mRNA from ACLF patients was remarkably higher than that from other patients (both < 0.05). The expression of SDF-l, CXCR4 and Ki67 from ACLF were the highest among the three groups (all < 0.01). The serum SDF-l levels in ACLF patients were significantly lower than that in other patients (both < 0.01). Moreover, in ACLF patients, the serum SDF-1 Levels were positively correlated with serum total bilirubin and international normalized ratio. In addition, the serum SDF-l levels in survival were significantly lower compared with the non-survivals ( < 0.05). The area under the curve for the serum SDF-1 level in predicting 28-d mortality was 0.722 ( < 0.05).
This study provides the SDF-1 changes in patients with HBV-related ACLF. The SDF-1 Level at admission may serve as a promising prognostic marker for predicting short-term prognosis.
基质细胞衍生因子-1（SDF-1）在干细胞募集至受损肝脏过程中起关键作用。然而，乙型肝炎病毒（HBV）相关慢加急性肝衰竭（ACLF）患者中SDF-1的变化尚待阐明。
研究HBV相关ACLF患者的SDF-1变化。
本研究纳入30例HBV相关ACLF患者、27例慢性乙型肝炎患者和20名健康个体。采用定量实时聚合酶链反应检测肝组织中SDF-1 mRNA表达。进行免疫组织化学染色以阐明SDF-1、CXC趋化因子受体4（CXCR4）和Ki67的表达。还采用酶联免疫吸附测定法检测血清SDF-1浓度。
ACLF患者的SDF-1 mRNA表达显著高于其他患者（均P<0.05）。ACLF患者的SDF-1、CXCR4和Ki67表达在三组中最高（均P<0.01）。ACLF患者的血清SDF-1水平显著低于其他患者（均P<0.01）。此外，在ACLF患者中，血清SDF-1水平与血清总胆红素和国际标准化比值呈正相关。另外，存活患者的血清SDF-1水平显著低于未存活患者（P<0.05）。血清SDF-1水平预测28天死亡率的曲线下面积为0.722（P<0.05）。
本研究提供了HBV相关ACLF患者的SDF-1变化情况。入院时的SDF-1水平可能作为预测短期预后的有前景的预后标志物。
