Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
Parasite. 2024;31:38. doi: 10.1051/parasite/2024038. Epub 2024 Jul 9.
In total, 901 dairy cow sera and data were collected from 51 farms in Nakhon Pathom, Ratchaburi and Kanchanaburi provinces (Western Region of Thailand). Serum samples were processed via the multispecies ELISA method to detect IgG antibodies against Toxoplasma gondii infection. The results demonstrated that the calculated true prevalence was 1.48% (95% CI, 0.64-2.75%) for the individual-level and 29.41% (95% CI, 18.71-43%) for the farm-level. The univariate risk factor analysis showed that the number of total owned cats, the presence of stray cats, and the frequency of cleaning per day were significant factors (p < 0.2). These three factors were subjected to logistic regression analysis, and the results revealed that the frequency of cleaning farms per day was a potential risk factor for T. gondii-seropositive farms (OR = 2.745, 95% CI, 1.15-8.69, p = 0.02). The frequency of cleaning might increase the T. gondii oocyst distribution within the barn area, thus increasing the possibility of infection. Our findings show that T. gondii continues to circulate in the dairy cow population in the western part of Thailand. The presence of cats on farms was not found to be associated with T. gondii infection, but the high frequency of cleaning the floor was, and contributed to the potential risk of infection.
本研究共从泰国西部那空巴统府、叻丕府和北碧府的 51 个农场收集了 901 份奶牛血清和数据。血清样本通过多物种 ELISA 方法进行处理，以检测针对弓形虫感染的 IgG 抗体。结果表明，个体水平的真实流行率为 1.48%（95%CI，0.64-2.75%），而农场水平的真实流行率为 29.41%（95%CI，18.71-43%）。单因素风险因素分析表明，总拥有猫的数量、流浪猫的存在以及每天清洁的频率是显著因素（p<0.2）。对这三个因素进行逻辑回归分析，结果表明，每天清洁农场的频率是 T. gondii 血清阳性农场的潜在风险因素（OR=2.745，95%CI，1.15-8.69，p=0.02）。每天清洁可能会增加谷仓区域内弓形虫卵囊的分布，从而增加感染的可能性。我们的研究结果表明，弓形虫继续在泰国西部的奶牛群体中传播。农场中猫的存在与 T. gondii 感染无关，但频繁清洁地面与感染的潜在风险有关。
