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血管活性肠肽前体编码基因的结构与表达

Structure and expression of the gene encoding the vasoactive intestinal peptide precursor.

作者信息

Linder S, Barkhem T, Norberg A, Persson H, Schalling M, Hökfelt T, Magnusson G

出版信息

Proc Natl Acad Sci U S A. 1987 Jan;84(2):605-9. doi: 10.1073/pnas.84.2.605.

DOI:10.1073/pnas.84.2.605
PMID:3025882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304259/
Abstract

The gene encoding the human vasoactive intestinal peptide (VIP) and the histidine-methionine amide (PHM-27) peptide hormone was isolated from lambda phage libraries. The human gene was found to be composed of seven exons spanning approximately 9 kilobase pairs. The first exon codes for an untranslated leader sequence, and the second exon codes for a putative signal peptide. DNA sequences coding for the VIP and PHM-27 hormones are located in two different exons. Southern blot analysis with genomic DNA suggested that a single copy of the VIP/PHM-27 gene is present in the human haploid genome. The expression of VIP/PHM-27 precursor mRNA in various tissues in the rat was analyzed by RNA gel blot hybridization. In the organs examined, expression was only detected in the brain and duodenum. RNA isolated from various regions of the rat brain--including the cortex, hypothalamus, and hippocampus--hybridized to both VIP- and PHM-27-specific probes. The same pattern of hybridization was found when VIP- and PHM-27-specific probes were used, suggesting that possible differences in the localization of VIP and PHM-27 peptides between different brain regions cannot be accounted for by differential RNA processing.

摘要

从λ噬菌体文库中分离出编码人血管活性肠肽（VIP）和组氨酸 - 甲硫氨酸酰胺（PHM - 27）肽激素的基因。发现该人类基因由七个外显子组成，跨度约为9千碱基对。第一个外显子编码一个非翻译的前导序列，第二个外显子编码一个假定的信号肽。编码VIP和PHM - 27激素的DNA序列位于两个不同的外显子中。用基因组DNA进行的Southern印迹分析表明，人单倍体基因组中存在一个VIP/PHM - 27基因拷贝。通过RNA凝胶印迹杂交分析了大鼠各种组织中VIP/PHM - 27前体mRNA的表达。在所检查的器官中，仅在脑和十二指肠中检测到表达。从大鼠脑的各个区域（包括皮质、下丘脑和海马体）分离的RNA与VIP和PHM - 27特异性探针都发生了杂交。当使用VIP和PHM - 27特异性探针时，发现了相同的杂交模式，这表明不同脑区之间VIP和PHM - 27肽定位的可能差异不能用不同的RNA加工来解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/86ff5235015d/pnas00267-0296-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/2f62f14d22dd/pnas00267-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/3329ab7f18a5/pnas00267-0295-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/a4ed3ed88bcf/pnas00267-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/76b8a059dc56/pnas00267-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/7d9e1e6d88c1/pnas00267-0296-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/86ff5235015d/pnas00267-0296-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/2f62f14d22dd/pnas00267-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/3329ab7f18a5/pnas00267-0295-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/a4ed3ed88bcf/pnas00267-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/76b8a059dc56/pnas00267-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/7d9e1e6d88c1/pnas00267-0296-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/304259/86ff5235015d/pnas00267-0296-d.jpg

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Structure and expression of the gene encoding the vasoactive intestinal peptide precursor.血管活性肠肽前体编码基因的结构与表达
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Biosynthesis of vasopressin, oxytocin, and neurophysins: isolation and characterization of two common precursors (propressophysin and prooxyphysin).血管加压素、催产素和神经垂体素的生物合成：两种常见前体（加压素神经垂体素原和催产素神经垂体素原）的分离与鉴定
Endocrinology. 1980 Dec;107(6):1880-91. doi: 10.1210/endo-107-6-1880.
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VIP in the rat brain: evidence for a major pathway linking the amygdala and hypothalamus via the stria terminalis.大鼠脑中的血管活性肠肽：通过终纹床核连接杏仁核和下丘脑的主要通路的证据。
Histochemistry. 1980 Feb;65(2):103-19. doi: 10.1007/BF00493159.
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Vasoactive intestinal polypeptide (VIP) in mouse and rat brain: an immunocytochemical study.
J Physiol. 2016 Nov 15;594(22):6463-6485. doi: 10.1113/JP272450. Epub 2016 Oct 5.
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Protected graft copolymer excipient leads to a higher acute maximum tolerated dose and extends residence time of vasoactive intestinal Peptide significantly better than sterically stabilized micelles.保护接枝共聚物赋形剂比空间稳定化胶束能显著提高血管活性肠肽的急性最大耐受剂量和延长其体内滞留时间。
Pharm Res. 2013 Mar;30(3):670-82. doi: 10.1007/s11095-012-0904-4. Epub 2012 Dec 7.
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The serendipitous origin of chordate secretin peptide family members.脊椎动物分泌素肽家族成员的偶然起源。
BMC Evol Biol. 2010 May 6;10:135. doi: 10.1186/1471-2148-10-135.
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Vasoactive intestinal peptide and the mammalian circadian system.血管活性肠肽与哺乳动物昼夜节律系统。
Gen Comp Endocrinol. 2007 Jun-Jul;152(2-3):165-75. doi: 10.1016/j.ygcen.2007.04.018. Epub 2007 May 26.
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Vasoactive intestinal peptide/peptide histidine isoleucine mRNA in the eye and suprachiasmatic nucleus of normal and monocularly enucleated rats.正常和单眼摘除大鼠眼睛及视交叉上核中血管活性肠肽/肽组氨酸异亮氨酸mRNA
Graefes Arch Clin Exp Ophthalmol. 1993 Sep;231(9):541-5. doi: 10.1007/BF00921120.
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Lineage-specific regulation of the vasoactive intestinal peptide gene in neuroblastoma cells is conferred by 5.2 kilobases of 5'-flanking sequence.神经母细胞瘤细胞中血管活性肠肽基因的谱系特异性调控由5.2千碱基的5'侧翼序列赋予。
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