Department of Internal Medicine, Osmangazi University Medical School, Eskisehir, Turkey.
Department of Hematology, Osmangazi University Medical School, Eskisehir, Turkey.
Clin Hemorheol Microcirc. 2024;88(2):289-296. doi: 10.3233/CH-242325.
Although plateletpheresis donation is commonly accepted as a safe procedure, its influence on platelet function, coagulation system and fibrinolysis is not completely elucidated.
In this study, we tried to assess the effects of plateletpheresis on donor's hemostasis system by measuring platelet activation, development of platelet-leukocyte aggregates, and coagulation activation.
Prospective observational study.
We used flow cytometry to determine the levels of platelet-monocyte complexes (PMC) and platelet-neutrophil complexes (PNC). sP-selectin and prothrombin fragment (PF) 1 + 2 values were determined by ELISA.
The PMC levels increased significantly seven days after apheresis in comparison with just after apheresis and 24 h after apheresis (p < 0.05). The PNC levels increased significantly seven days after apheresis compared to immediately after apheresis (p < 0.05). sP-selectin values decreased significantly immediately after apheresis (p < 0.05). While sP-selectin values increased seven days after apheresis in comparison with immediately after apheresis and 24 h after apheresis, but there were not statistically significant differences for sP-selectin levels (p > 0.05). PF1 + 2 levels decreased significantly immediately after apheresis compared to pre-apheresis (p < 0.05) and increased 24 h after apheresis and seven days after apheresis, but these differences were not statistically significant.
We concluded that plateletpheresis affects platelet activation but does not cause any change in coagulation activation.
尽管血小板单采术被普遍认为是一种安全的程序，但它对血小板功能、凝血系统和纤溶系统的影响尚未完全阐明。
本研究旨在通过检测血小板活化、血小板-白细胞聚集体的形成以及凝血激活，评估血小板单采术对供者止血系统的影响。
前瞻性观察研究。
我们使用流式细胞术来确定血小板-单核细胞复合物（PMC）和血小板-中性粒细胞复合物（PNC）的水平。通过 ELISA 测定 sP-选择素和凝血酶原片段 1+2（PF1+2）的含量。
与单采术后即刻和 24 小时相比，单采术后 7 天 PMC 水平显著升高（p<0.05）。与单采术后即刻相比，单采术后 7 天 PNC 水平显著升高（p<0.05）。sP-选择素水平在单采术后即刻显著降低（p<0.05）。单采术后 7 天 sP-选择素水平与即刻和 24 小时相比升高，但差异无统计学意义（p>0.05）。与单采术前相比，单采术后即刻 PF1+2 水平显著降低（p<0.05），单采术后 24 小时和 7 天 PF1+2 水平升高，但差异无统计学意义。
我们得出结论，血小板单采术影响血小板活化，但不会引起凝血激活的任何变化。
