Department of Hematology, Koç University Medical School , İstanbul , Turkey.
Department of Internal Medicine, Eskişehir Osmangazi University Medical School , Eskisehir , Turkey.
Platelets. 2019;30(8):989-993. doi: 10.1080/09537104.2018.1542126. Epub 2018 Nov 29.
Since there is still debate on the effects of plateletpheresis on coagulation system, we aimed to perform a global assessment of donor's hemostatic function undergoing plateletpheresis by rotation thromboelastometry (ROTEM) analysis and to clarify if plateletpheresis procedure induces a hypercoagulable state. Thirty male plateletpheresis donors were included in the study. Four blood samples were drawn at different time intervals: before the beginning of the apheresis procedure; immediately after the completion of the apheresis procedure; 24 h and 7 days after the apheresis procedure. "Hypercoagulability" was diagnosed readily by having an accelerated clot formation, as evidenced by shortening of CFT and an increase of the clot strength, as evidenced by increasing of MCF. In INTEM assay, CFT value after apheresis was significantly prolonged compared with baseline value while CFT value 7 days after apheresis was significantly shortened compared with values immediately and 24 h after apheresis ( < 0.001). However, CFT-INTEM still did not show any shortening in any of the measurements when compared to pre-apheresis value. MCF value after apheresis was significantly shortened compared with baseline value while MCF value 7 days after apheresis was significantly prolonged compared with values immediately and 24 h after apheresis ( < 0.001). However, MCF-INTEM still did not show any increase in any of the measurements when compared to pre-apheresis value. There was no significant difference in CT value between four measurements ( = 0.064). In EXTEM assay, CFT value after apheresis was significantly prolonged compared with baseline value while CFT value 7 days after apheresis was significantly shortened compared with values immediately and 24 h after apheresis ( < 0.001). However, CFT-EXTEM still did not show any shortening in any of the measurements when compared to pre-apheresis value. MCF values immediately and 24 h after apheresis were significantly shortened compared with baseline value while MCF value 7 days after apheresis was significantly prolonged compared with values immediately and 24 h after apheresis ( < 0.001). However, MCF-EXTEM still did not show any increase in any of the measurements when compared to pre-apheresis value. We found no differences in CT value between four measurements ( = 0.208). Since ROTEM tracings on both INTEM and EXTEM assays did not reveal any significant shortening of CFT and increasing of MCF in any of the measurements after apheresis procedure, we concluded that plateletpheresis does not induce a hypercoagulable state in healthy donors.
由于目前对于血小板单采术对凝血系统的影响仍存在争议，我们旨在通过旋转血栓弹性描记术（ROTEM）分析，对行血小板单采术的供者止血功能进行全面评估，并阐明血小板单采术是否会导致高凝状态。本研究纳入了 30 名男性血小板单采术供者。在不同的时间间隔采集了 4 份血样：在单采术开始前；单采术完成后即刻；单采术后 24 小时和 7 天。“高凝状态”很容易被诊断，表现为凝血块形成加速，这表现为 CFT 缩短和 MCF 增加。在 INTEM 检测中，与基线值相比，血小板单采术后的 CFT 值显著延长，而血小板单采术后 7 天的 CFT 值与即刻和 24 小时后相比显著缩短（<0.001）。然而，与术前值相比，CFT-INTEM 在任何测量中均未显示缩短。与基线值相比，血小板单采术后的 MCF 值显著缩短，而血小板单采术后 7 天的 MCF 值与即刻和 24 小时后相比显著延长（<0.001）。然而，与术前值相比，CFT-INTEM 在任何测量中均未显示增加。在 EXTEM 检测中，与基线值相比，血小板单采术后的 CFT 值显著延长，而血小板单采术后 7 天的 CFT 值与即刻和 24 小时后相比显著缩短（<0.001）。然而，与术前值相比，CFT-EXTEM 在任何测量中均未显示缩短。与基线值相比，血小板单采术后即刻和 24 小时的 MCF 值显著缩短，而血小板单采术后 7 天的 MCF 值与即刻和 24 小时后相比显著延长（<0.001）。然而，与术前值相比，CFT-EXTEM 在任何测量中均未显示增加。在 4 次测量之间，CT 值没有差异（=0.208）。由于在 INTEM 和 EXTEM 检测中，血小板单采术后的 ROTEM 描记均未显示在任何测量中 CFT 显著缩短和 MCF 增加，因此我们得出结论，血小板单采术不会导致健康供者的高凝状态。
