Warren R H, Gordon E, Azarnia R
Eur J Cell Biol. 1985 Sep;38(2):245-53.
Tropomyosin distribution has been studied in two normal lines and one transformed line of rat kidney cells during the early phases of substrate attachment and growth. One non-motile normal line, which spreads rapidly after attachment, immediately begins to assemble prominent stress fibers that contain tropomyosin. It displays small peripheral ruffles that are not noticeably stained with anti-tropomyosin. The other normal line is motile and produces large ruffles that are brightly stained with anti-tropomyosin. Large numbers of tropomyosin-positive stress fibers assemble only after the cells stop moving and lose the peripheral ruffles. The transformed line does not assemble stress fibers but does contain large numbers of actin filament bundles in ruffles on the cell surface that are stained with anti-tropomyosin. These observations indicate that cytoskeletal tropomyosin is not restricted in distribution to stress fibers, and may undergo re-organization along with actin during the transition from motile to non-motile behavior.
在底物附着和生长的早期阶段，对大鼠肾细胞的两条正常系和一条转化系中的原肌球蛋白分布进行了研究。一条不运动的正常系，附着后迅速铺展，立即开始组装含有原肌球蛋白的明显应力纤维。它显示出小的周边褶皱，用抗原肌球蛋白染色不明显。另一条正常系是运动性的，产生大的褶皱，用抗原肌球蛋白染色明亮。只有在细胞停止移动并失去周边褶皱后，大量原肌球蛋白阳性应力纤维才会组装。转化系不组装应力纤维，但在细胞表面用抗原肌球蛋白染色的褶皱中确实含有大量肌动蛋白丝束。这些观察结果表明，细胞骨架原肌球蛋白的分布不限于应力纤维，并且在从运动行为向非运动行为转变期间可能与肌动蛋白一起发生重组。
