Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China; Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Liver Transplant Center, Organ Transplant Center, West China Hospital of Sichuan University, Chengdu, China.
Biomaterials. 2024 Dec;311:122691. doi: 10.1016/j.biomaterials.2024.122691. Epub 2024 Jul 1.
Acoustic holography (AH), a promising approach for cell patterning, emerges as a powerful tool for constructing novel invitro 3D models that mimic organs and cancers features. However, understanding changes in cell function post-AH remains limited. Furthermore, replicating complex physiological and pathological processes solely with cell lines proves challenging. Here, we employed acoustical holographic lattice to assemble primary hepatocytes directly isolated from mice into a cell cluster matrix to construct a liver-shaped tissue sample. For the first time, we evaluated the liver functions of AH-patterned primary hepatocytes. The patterned model exhibited large numbers of self-assembled spheroids and superior multifarious core hepatocyte functions compared to cells in 2D and traditional 3D culture models. AH offers a robust protocol for long-term in vitro culture of primary cells, underscoring its potential for future applications in disease pathogenesis research, drug testing, and organ replacement therapy.
声全息术 (AH) 是一种有前途的细胞图案化方法，可用于构建模仿器官和癌症特征的新型体外 3D 模型，是一种强大的工具。然而，对于 AH 后细胞功能的变化的理解仍然有限。此外，仅用细胞系复制复杂的生理和病理过程具有挑战性。在这里，我们采用声全息晶格将直接从小鼠中分离的原代肝细胞组装成细胞簇基质，以构建肝状组织样本。我们首次评估了声全息图案化原代肝细胞的肝脏功能。与 2D 和传统 3D 培养模型中的细胞相比，该图案模型表现出大量的自组装球体和更优越的多种核心肝细胞功能。AH 为原代细胞的长期体外培养提供了一种强大的方案，突出了其在疾病发病机制研究、药物测试和器官替代治疗中的潜在应用。
