Kobayashi K, Tomonaga S, Teshima K, Kajii T
Eur J Immunol. 1985 Sep;15(9):952-6. doi: 10.1002/eji.1830150916.
We identified the presence in the Aleutian skate, Bathyraja aleutica, of two classes of immunoglobulins (Ig), a high molecular weight Ig analogous to mammalian IgM and a low molecular weight Ig, similarly to the spiny rasp skate, Raja kenojei, (Kobayashi, K. et al., Mol. Immunol. 1984. 21: 397), using an immunological cross-reaction with the specific antisera to the spiny rasp skate Ig components. The antigenic similarity of the heavy chains of the Ig of the Aleutian skate and those of the spiny rasp skate was less than that between their light chains. Two types of Ig-producing cells, one producing the high molecular weight and the other forming the low molecular weight Ig, were present in the spleen of embryos and adults in the Aleutian skate at a ratio of 5-6:4-5. The number of these Ig-producing cells increased with advancing development of the embryos but was 1/20 to 1/50 of those of adults. Cells, each of which were capable of forming both classes of Ig, were found in the spleen of embryos but not in that of adults. These results suggested that the spleen of the Aleutian skate is the primary lymphoid organ for B lymphocyte differentiation and proliferation, possibly equivalent to the bursa of birds.
我们利用与多棘锉鳐（Raja kenojei）Ig成分的特异性抗血清的免疫交叉反应，确定了阿留申鳐（Bathyraja aleutica）中存在两类免疫球蛋白（Ig），一类是类似于哺乳动物IgM的高分子量Ig，另一类是低分子量Ig，这与多棘锉鳐的情况类似（小林K.等人，《分子免疫学》，1984年，21卷：397页）。阿留申鳐Ig重链与多棘锉鳐Ig重链的抗原相似性低于它们轻链之间的相似性。在阿留申鳐胚胎和成体的脾脏中存在两种类型的Ig产生细胞，一种产生高分子量Ig，另一种产生低分子量Ig，其比例为5 - 6:4 - 5。这些Ig产生细胞的数量随着胚胎发育的推进而增加，但仅为成体的1/20至1/50。在胚胎脾脏中发现了能够产生两类Ig的细胞，但在成体脾脏中未发现。这些结果表明，阿留申鳐的脾脏是B淋巴细胞分化和增殖的主要淋巴器官，可能相当于鸟类的法氏囊。
