Tomonaga S, Kobayashi K, Kajii T, Awaya K
Dev Comp Immunol. 1984 Fall;8(4):803-12. doi: 10.1016/0145-305x(84)90063-6.
We recently reported the presence in the skate, Raja kenojei, of two immunoglobulins, a high molecular weight immunoglobulin analogous to mammalian IgM, and a low molecular weight immunoglobulin belonging to a hitherto undescribed class. In view of these findings, we studied lymphoid organs of the skate using morphological and immunocytochemical means. The spleen and the intestinal mucosa had numerous plasma cells and lymphocytes, while the Leydig organ of the esophagus, the epigonal organ and the liver contained these cells in a much lesser frequency. Immunocytochemical studies proved these plasma cells and some of the lymphocytes to be immunoglobulin-forming cells. Double immunofluorescence staining of the spleen and other lymphoid tissues demonstrated the occurrence at a 1: 1 ratio of two distinct populations of cells, one forming the high molecular weight immunoglobulin and the other producing the low molecular weight immunoglobulin.
我们最近报道称，在孔鳐（Raja kenojei）体内存在两种免疫球蛋白，一种是类似于哺乳动物IgM的高分子量免疫球蛋白，另一种是属于迄今未描述类别的低分子量免疫球蛋白。鉴于这些发现，我们使用形态学和免疫细胞化学方法研究了孔鳐的淋巴器官。脾脏和肠黏膜中有大量浆细胞和淋巴细胞，而食管的莱迪希氏器官、生殖腺器官和肝脏中这些细胞的数量则少得多。免疫细胞化学研究证明这些浆细胞和一些淋巴细胞是形成免疫球蛋白的细胞。对脾脏和其他淋巴组织进行的双重免疫荧光染色显示，两种不同细胞群以1:1的比例出现，一种形成高分子量免疫球蛋白，另一种产生低分子量免疫球蛋白。
