Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (URL-CNRST 05), Université Cadi Ayyad, Marrakech, Morocco.
Laboratoire de Biotechnologie Microbienne et Molécules Bioactives, Faculté des Sciences et Techniques, Université Sidi Mohamed Ben Abdellah, Fès, Morocco.
Int J Biol Macromol. 2024 Sep;276(Pt 1):133800. doi: 10.1016/j.ijbiomac.2024.133800. Epub 2024 Jul 10.
The use of beneficial microorganisms and polysaccharides for the biocontrol of plant diseases currently represents a promising tool for the management of soil-borne pathogens. Despite advancements, enhancing the efficacy and sustainability of these biocontrol methods, particularly in complex soil environments, remains a challenge. Thus, we investigated the potential of four PGPR strains encapsulated in natural alginate extracted from a brown seaweed Bifurcaria bifurcata to evaluate its biocontrol capacities against Verticillium wilt of tomato, ensuring optimal performance through a synergistic effect and innovative bacterial release. Our research demonstrated that the application of PGPR and alginate reduced disease severity and mortality rate and increased the natural defenses of tomato. Results showed that supplying alginate or the PGPR consortium at the root level s stimulates phenylalanine ammonia-lyase activity (the key enzyme of the phenylpropanoid metabolism) and the accumulation of phenolic compounds and lignin in leaves and roots. Treatment with PGPR encapsulated in alginate beads showed the best biocontrol efficiency and was accompanied by a synergistic effect reflecting a rapid, intense, and systemic induction of defense mechanisms known for their effectiveness in inducing resistance in tomato. These promising results suggest that such bioformulations could lead to innovative agricultural practices for sustainable plant protection against pathogens.
利用有益微生物和多糖来生物防治植物病害，目前是一种有前途的管理土传病原体的工具。尽管已经取得了进展，但提高这些生物防治方法的效果和可持续性，特别是在复杂的土壤环境中，仍然是一个挑战。因此，我们研究了四种 PGPR 菌株在天然褐藻海藻酸中的包封潜力，以评估其对番茄黄萎病的生物防治能力，通过协同作用和创新的细菌释放来确保最佳性能。我们的研究表明，PGPR 和海藻酸盐的应用降低了番茄黄萎病的严重程度和死亡率，并增强了番茄的天然防御能力。结果表明，在根部水平供应海藻酸盐或 PGPR 联合体刺激苯丙氨酸解氨酶活性（苯丙烷代谢的关键酶）和叶片和根部中酚类化合物和木质素的积累。用海藻酸盐珠粒包封的 PGPR 处理显示出最好的生物防治效率，并伴随着协同作用，反映了防御机制的快速、强烈和系统诱导，这些机制在诱导番茄抗性方面非常有效。这些有希望的结果表明，这种生物制剂可能会导致创新的农业实践，以实现可持续的植物保护，防止病原体的侵害。
