Laboratoire d'Agrobiotechnologie et Bioingénierie, Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakesh 40000, Morocco.
Institut Pascal, Université Clermont Auvergne, CNRS, SIGMA Clermont, F-63000 Clermont-Ferrand, France.
Mar Drugs. 2020 Nov 26;18(12):596. doi: 10.3390/md18120596.
Fucoidans from Moroccan brown seaweed and were tested for their elicitor activity after their purification and complete characterization. The fucoidans of (BBF) and of (FSF) were extracted and purified then characterized by infrared spectroscopy, proton nuclear magnetic resonance spectroscopy and size exclusion chromatography. The results show that BBF and FSF are mainly sulfated with 45.49 and 49.53% (/) sulfate, respectively. Analysis of neutral sugars determined by gas chromatography-mass spectrometry showed that FSF and BBF were mainly composed of 64% and 91% fucose and 20% and 6% galactose, respectively, with a few other sugars such as glucose (8% in FSF), rhamnose (1% in BBF) and mannose (8% in FSF and, 2% in BBF). The eliciting activity of these sulfated polysaccharides in stimulating the natural defenses of the date palm was evaluated through the activity of phenylalanine ammonia-lyase (PAL), and the increase in phenols and lignin content in the roots. The results obtained clearly show that the two fucoidans early and intensely stimulate the natural defenses of the date palm after 24 h of treatments. This remarkable elicitor effect seems to be linked to the sulfated groups compared to non-sulfate alginates extracted from the same algae. These results open promising perspectives for a biological control approach against date palm diseases.
从摩洛哥褐藻和中提取并纯化了褐藻胶，并对其进行了完全表征，以测试其诱导活性。（BBF）和（FSF）的褐藻胶被提取和纯化，然后通过红外光谱、质子核磁共振波谱和凝胶渗透色谱进行表征。结果表明，BBF 和 FSF 主要带有 45.49%和 49.53%（/）硫酸酯基。通过气相色谱-质谱分析中性糖的结果表明，FSF 和 BBF 主要由 64%和 91%的岩藻糖和 20%和 6%的半乳糖组成，还有一些其他的糖，如 FSF 中的葡萄糖（8%）、BBF 中的鼠李糖（1%）和 FSF 中的甘露糖（8%）和 BBF 中的甘露糖（2%）。通过苯丙氨酸解氨酶（PAL）活性和根系中酚类和木质素含量的增加来评估这些硫酸化多糖在刺激油棕天然防御中的诱导活性。研究结果清楚地表明，两种褐藻胶在处理 24 小时后，能早期、强烈地刺激油棕的天然防御。这种显著的诱导效应似乎与与从同一藻类中提取的非硫酸盐藻酸盐相比的硫酸基团有关。这些结果为油棕病害的生物防治方法开辟了广阔的前景。
