Pereira-Smith O M, Fisher S F, Smith J R
Exp Cell Res. 1985 Oct;160(2):297-306. doi: 10.1016/0014-4827(85)90177-6.
Cytoplasts derived from senescent and quiescent human diploid cells inhibit DNA synthesis initiation when fused with cells capable of proliferation. When the cytoplasts were subjected to a variety of conditions (trypsin and cycloheximide treatment and growth on fibronectin), this inhibitory activity was lost, suggesting that the inhibitors involved were proteins associated with the surface membranes of the cells. We have studied the quiescent cell inhibitor in greater detail and determined that surface membrane-enriched preparations isolated from quiescent cells and proteins extracted from these membrane preparations have DNA synthesis-inhibitory activity.
源自衰老和静止的人二倍体细胞的细胞质体与能够增殖的细胞融合时，会抑制DNA合成起始。当细胞质体经受各种条件（胰蛋白酶和环己酰亚胺处理以及在纤连蛋白上生长）时，这种抑制活性丧失，这表明所涉及的抑制剂是与细胞表面膜相关的蛋白质。我们更详细地研究了静止细胞抑制剂，并确定从静止细胞中分离出的富含表面膜的制剂以及从这些膜制剂中提取的蛋白质具有DNA合成抑制活性。
