Biosensor Development Group, Department of Biotechnology & Food Technology, Punjabi University Patiala, 147002, Punjab, India.
Department of Biotechnology, Mata Gujri College Fatehgarh Sahib-140407, Punjab, India.
Anal Biochem. 2024 Nov;694:115616. doi: 10.1016/j.ab.2024.115616. Epub 2024 Jul 11.
Chlorogenic acid, a phenolic compound, is prevalent across various plant species and has been known for its pharmacological advantages. Health care experts have identified chlorogenic acid as a potential biomarker for treatment of a wide range of illnesses. Therefore, achieving efficient extraction and analysis of chlorogenic acid from plants and their products has become essential. Molecularly imprinted polymers (MIPs) are highly effective adsorbent for the extraction of chlorogenic acid from complex matrices. Currently, there is a lack of comprehensive review article that consolidate the methods utilized for the purification of chlorogenic acid through molecular imprinting. In this context, we have surveyed the common approaches employed in preparing MIPs specifically designed for the analysis of chlorogenic acid, including both conventional and newly developed. This review discusses the advantages, limitations of polymerization techniques and proposed strategies to produce more efficient MIPs for chlorogenic acid enrichment in complex samples. Additionaly, we present advanced imprinting methods for designing MIPs, which improve the adsorption capacity, sensitivity and selectivity towards chlorogenic acid.
绿原酸是一种酚类化合物，广泛存在于各种植物物种中，具有药理学优势。医疗保健专家已经确定绿原酸是治疗多种疾病的潜在生物标志物。因此，从植物及其产品中高效提取和分析绿原酸变得至关重要。分子印迹聚合物（MIPs）是从复杂基质中提取绿原酸的高效吸附剂。目前，缺乏全面的综述文章来整合通过分子印迹法纯化绿原酸所使用的方法。在这方面，我们调查了为分析绿原酸而专门设计的 MIPs 的常见制备方法，包括传统和新开发的方法。本综述讨论了聚合技术的优点、局限性以及提出的策略，以生产更有效的 MIPs，用于从复杂样品中富集绿原酸。此外，我们还介绍了用于设计 MIPs 的先进印迹方法，这些方法提高了对绿原酸的吸附容量、灵敏度和选择性。
