van Wezel A L, van der Velden-de Groot C A, de Haan H H, van den Heuvel N, Schasfoort R
Dev Biol Stand. 1985;60:229-36.
Through the developments in molecular biology the interest for large scale animal cell cultivation has sharply increased during the last 5 years. At our laboratory, four different cultivation systems were studied, all of which are homogeneous culture systems, as they lend themselves best for scaling up and for the control of culture conditions. The four different systems which were compared are: batch culture, continuous chemostat, continuous recycling and continuous perfusion culture system, both for cells growing in suspension and for anchorage dependent cells in microcarrier culture. Our results indicate that for the production of virus vaccines and cells the batch and recycling culture system are most suitable. Disadvantages of the continued chemostat culture system are: the system is only applicable for cells growing in suspension; relatively low concentrations of cells and cellular products are obtained. The continuous perfusion system appears to be very suitable for the production of cellular components and also for the production of viruses which do not give cell lysis.
在过去五年中，随着分子生物学的发展，大规模动物细胞培养的关注度急剧上升。在我们实验室，研究了四种不同的培养系统，它们均为均匀培养系统，因为它们最适合扩大规模和控制培养条件。所比较的四种不同系统分别是：分批培养、连续恒化器培养、连续循环培养和连续灌注培养系统，适用于悬浮生长的细胞以及微载体培养中依赖贴壁的细胞。我们的结果表明，对于病毒疫苗和细胞的生产，分批培养和循环培养系统最为合适。连续恒化器培养系统的缺点是：该系统仅适用于悬浮生长的细胞；获得的细胞和细胞产物浓度相对较低。连续灌注系统似乎非常适合细胞成分的生产，也适合生产不会导致细胞裂解的病毒。
