Clarke J B, Griffiths J B
Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wilts., UK.
Cytotechnology. 1990 Sep;4(2):145-53. doi: 10.1007/BF00365095.
In an attempt to establish a bioreactor system for generation of HIV that is practicable, efficient, biologically contained, and capable of scale up, the production of two strains of this virus was examined in suspension culture and the 'Porosphere' fixed bed system. HIV 1 and HIV 2 were grown successfully in both these types of reactor. The porosphere reactor theoretically appears to offer a better environment for HIV production, but evidence for significantly improved yields from this system, compared to suspension, was equivocal. However, this configuration facilitated media changes during culture. The data clearly showed that the culture system and cell environment significantly affected cell-virus interrelationships. Switches between lytic--and persistent--type infections, and changes in the virus population were observed.
为建立一个可行、高效、生物安全且能够扩大规模的用于产生HIV的生物反应器系统，在悬浮培养和“Porosphere”固定床系统中对该病毒的两种毒株的生产进行了研究。HIV-1和HIV-2在这两种类型的反应器中均成功生长。从理论上讲，Porosphere反应器似乎为HIV的生产提供了更好的环境，但与悬浮培养相比，该系统产量显著提高的证据并不明确。然而，这种配置便于在培养过程中更换培养基。数据清楚地表明，培养系统和细胞环境显著影响细胞与病毒的相互关系。观察到了溶细胞型感染和持续感染之间的转换以及病毒群体的变化。
