Lazar A, Silverstein L, Margel S, Mizrahi A
Dev Biol Stand. 1985;60:457-65.
A new microcarrier (MC) culturing system for anchorage-dependent cells is described. The system is based on polyacrylein microspheres encapsulated in agarose and derivatized by various amino ligands. In practice, any protein or ligand with primary amino groups may be covalently bound to the microspheres through their aldehyde groups. The ease with which the beads may be derivatized enables the preparation of MC with desired surface properties for growing a variety of cells. In this model system the beads were derivatized by 2-(diethylamino)ethylamine (DEAE), diaminohexane (DAH), poly-L-lysine (PL), gelatin and collagen. The derivatized beads supported the growth of primary cells, diploid cell strains and established cell lines for biological studies as well as for production of various cell products.
本文描述了一种用于贴壁依赖性细胞的新型微载体（MC）培养系统。该系统基于包裹在琼脂糖中并通过各种氨基配体衍生化的聚丙烯微球。实际上，任何具有伯氨基的蛋白质或配体都可以通过其醛基与微球共价结合。微球易于衍生化，这使得能够制备具有所需表面性质的微载体，以培养多种细胞。在这个模型系统中，微球用2-（二乙氨基）乙胺（DEAE）、二氨基己烷（DAH）、聚-L-赖氨酸（PL）、明胶和胶原蛋白进行了衍生化。衍生化的微球支持原代细胞、二倍体细胞株和已建立的细胞系的生长，可用于生物学研究以及各种细胞产物的生产。
