Food and Health Function Research Center, Ehime University, Matsuyama 790-8566, Japan.
Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama 790-8566, Japan.
Nutrients. 2024 Jun 29;16(13):2080. doi: 10.3390/nu16132080.
This study aimed to investigate a synergistic anti-inflammatory effect of a citrus flavonoid nobiletin and docosahexaenoic acid (DHA), one of -3 long-chain polyunsaturated fatty acids, in combination. Simultaneous treatment with nobiletin and DHA synergistically inhibited nitric oxide production (combination index < 0.9) by mouse macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS) without cytotoxicity. On the other hand, the inhibitory effect of nobiletin and DHA in combination on proinflammatory cytokine production was not synergistic. Neither nobiletin nor DHA affected the phagocytotic activity of RAW 264.7 cells stimulated with LPS. Immunoblot analysis revealed that the inhibition potency of DHA on the phosphorylation of ERK and p38 and nuclear translocation of NF-κB is markedly enhanced by simultaneously treating with nobiletin, which may lead to the synergistic anti-inflammatory effect. Overall, our findings show the potential of the synergistic anti-inflammatory effect of nobiletin and DHA in combination.
本研究旨在探讨柑橘类黄酮诺必特与二十二碳六烯酸（DHA）的协同抗炎作用，DHA 是一种 -3 长链多不饱和脂肪酸。同时用诺必特和 DHA 处理可协同抑制脂多糖（LPS）刺激的鼠巨噬细胞样 RAW 264.7 细胞一氧化氮的产生（组合指数<0.9），而无细胞毒性。另一方面，诺必特和 DHA 的组合对促炎细胞因子产生的抑制作用不是协同的。诺必特和 DHA 均不影响 LPS 刺激的 RAW 264.7 细胞的吞噬活性。免疫印迹分析表明，同时用诺必特处理可显著增强 DHA 对 ERK 和 p38 的磷酸化以及 NF-κB 核易位的抑制作用，这可能导致协同抗炎作用。总的来说，我们的研究结果表明诺必特和 DHA 联合使用具有协同抗炎作用的潜力。
