NICM Health Research Institute, Western Sydney University, Westmead, NSW 2145, Australia.
Pharmacology Unit, School of Medicine, Western Sydney University, Narellan Road & Gilchrist Drive, Campbelltown, NSW 2560, Australia.
Molecules. 2022 Jun 16;27(12):3877. doi: 10.3390/molecules27123877.
This study aims to investigate the combined anti-inflammatory activity of ginger and turmeric extracts. By comparing the activities of individual and combined extracts in lipopolysaccharide and interferon-γ-induced murine RAW 264.7 cells, we demonstrated that ginger-turmeric combination was optimal at a specific ratio (5:2, w/w) in inhibiting nitric oxide, tumour necrosis factor and interleukin 6 with synergistic interaction (combination index < 1). The synergistic inhibitory effect on TNF was confirmed in human monocyte THP-1 cells. Ginger-turmeric combination (5:2, w/w) also upregulated nuclear factor erythroid 2−related factor 2 activity and heme oxygenase-1 protein expression. Additionally, 6-shogaol, 8-shogaol, 10-shogaol and curcumin were the leading compounds in reducing major proinflammatory mediators and cytokines, and a simplified compound combination of 6-s, 10-s and curcumin showed the greatest potency in reducing LPS-induced NO production. Our study provides scientific evidence in support of the combined use of ginger and turmeric to alleviate inflammatory processes.
本研究旨在探究生姜和 turmeric 提取物的联合抗炎活性。通过比较 LPS 和 IFN-γ诱导的 RAW 264.7 细胞中单个和组合提取物的活性，我们证明在特定比例（5:2，w/w）下，生姜- turmeric 组合在抑制一氧化氮、肿瘤坏死因子和白细胞介素 6 方面具有最佳的协同作用（CI<1）。在人单核细胞 THP-1 细胞中也证实了对 TNF 的协同抑制作用。生姜- turmeric 组合（5:2，w/w）还上调了核因子红细胞 2-相关因子 2 活性和血红素加氧酶-1 蛋白表达。此外，6-姜酚、8-姜酚、10-姜酚和姜黄素是减少主要促炎介质和细胞因子的主要化合物，而 6-s、10-s 和姜黄素的简化化合物组合在减少 LPS 诱导的 NO 产生方面显示出最大的效力。本研究为支持生姜和 turmeric 的联合使用以缓解炎症过程提供了科学证据。
