Department of Pharmacology and Toxicology, Heersink School of Medicine, The University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Department of Physiology & Biophysics, Howard University College of Medicine, Washington, DC 20059, USA.
Int J Mol Sci. 2024 Jun 27;25(13):7021. doi: 10.3390/ijms25137021.
DNA damage in the brain is influenced by endogenous processes and metabolism along with exogenous exposures. Accumulation of DNA damage in the brain can contribute to various neurological disorders, including neurodegenerative diseases and neuropsychiatric disorders. Traditional methods for assessing DNA damage in the brain, such as immunohistochemistry and mass spectrometry, have provided valuable insights but are limited by their inability to map specific DNA adducts and regional distributions within the brain or genome. Recent advancements in DNA damage detection methods offer new opportunities to address these limitations and further our understanding of DNA damage and repair in the brain. Here, we review emerging techniques offering more precise and sensitive ways to detect and quantify DNA lesions in the brain or neural cells. We highlight the advancements and applications of these techniques and discuss their potential for determining the role of DNA damage in neurological disease.
脑内的 DNA 损伤受到内源性过程和代谢以及外源性暴露的影响。脑内 DNA 损伤的积累可能导致各种神经疾病，包括神经退行性疾病和神经精神疾病。评估脑内 DNA 损伤的传统方法，如免疫组织化学和质谱分析，提供了有价值的见解，但受到其无法绘制脑内或基因组中特定 DNA 加合物和区域分布的限制。最近在 DNA 损伤检测方法方面的进展为解决这些限制并进一步了解脑内 DNA 损伤和修复提供了新的机会。在这里，我们回顾了新兴技术，这些技术提供了更精确和敏感的方法来检测和量化脑或神经细胞中的 DNA 损伤。我们强调了这些技术的进步和应用，并讨论了它们在确定 DNA 损伤在神经疾病中的作用方面的潜力。
