Department of Obstetrics and Perinatology, Medical University of Lublin, 20-090 Lublin, Poland.
Institute of Medical Science, College of Medical Science, University of Rzeszow, 35-959 Rzeszow, Poland.
Int J Mol Sci. 2024 Jun 27;25(13):7040. doi: 10.3390/ijms25137040.
Gestational diabetes mellitus (GDM) is an intolerance of carbohydrate of any degree, which appears for the first time or is diagnosed during pregnancy. The objective of this study is to assess the differences in circular RNA (circRNA) in a Polish pregnant population with and without GDM. A total of 62 pregnant women, 34 with GDM and 28 controls, were enrolled in the study. Total RNAs were extracted from plasma and reverse transcription to complementary DNA (cDNA) was performed. A panel covering 271 amplicons, targeting both linear and circular as well as negative control gene transcripts, was used. Next-generation sequencing was used to evaluate the circRNA quantity. Data analysis was performed using the Coverage Analysis plugin in the Torrent Suite Software (Torrent Suite 5.12.3). A two-step normalization was performed by dividing each transcript read count by the total number of reads generated for the sample, followed by dividing the quantity of each transcript by β-actin gene expression. Both circular and linear forms of RNAs were independently evaluated. A total of 57 transcripts were dysregulated between pregnant women with GDM and controls. Most of the targets (n = 25) were downregulated (cut-off ratio below 0.5), and one target showed a trend toward strong upregulation (ratio 1.45). A total of 39 targets were positively correlated with fasting plasma glucose (FPG), but none of the tested targets were correlated with insulin, CRP or HOMA-IR levels. Among the pregnant women with gestational diabetes, the relative quantity of hsa_circ_0002268 () was approximately 120% higher than among healthy pregnant women: 0.046 [0.022-0.096] vs. 0.021 [0.007-0.047], respectively, ( = 0.0029). Elevated levels of hsa_circ_0002268 might be specific to the Polish population of pregnant women with GDM, making it useful as a potential molecular biomarker in the management of GDM in Poland.
妊娠期糖尿病（GDM）是一种任何程度的碳水化合物不耐受，它在怀孕期间首次出现或被诊断出来。本研究的目的是评估波兰有和没有 GDM 的孕妇群体之间环状 RNA（circRNA）的差异。总共纳入了 62 名孕妇，其中 34 名患有 GDM，28 名为对照组。从血浆中提取总 RNA 并进行逆转录以合成互补 DNA（cDNA）。使用了一个涵盖 271 个扩增子的面板，靶向线性和环状以及负控基因转录物。使用下一代测序来评估 circRNA 的数量。数据分析使用 Torrent Suite 软件（Torrent Suite 5.12.3）中的 Coverage Analysis 插件进行。通过将每个转录本的读取计数除以样品生成的总读取数，然后将每个转录本的数量除以 β-肌动蛋白基因的表达，对每个转录本的数量进行了两步归一化。分别评估了环状和线性 RNA。在患有 GDM 的孕妇和对照组之间，共有 57 个转录本存在差异表达。大多数靶标（n = 25）下调（低于 0.5 的比值），一个靶标表现出强烈上调的趋势（比值 1.45）。共有 39 个靶标与空腹血浆葡萄糖（FPG）呈正相关，但没有一个测试靶标与胰岛素、CRP 或 HOMA-IR 水平相关。在患有妊娠期糖尿病的孕妇中，hsa_circ_0002268（）的相对数量比健康孕妇高约 120%：0.046 [0.022-0.096] 与 0.021 [0.007-0.047]，分别，（=0.0029）。hsa_circ_0002268 的升高水平可能是波兰患有 GDM 的孕妇群体所特有的，使其成为波兰 GDM 管理中潜在的分子生物标志物。
