Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
Int J Mol Sci. 2024 Jun 28;25(13):7152. doi: 10.3390/ijms25137152.
4'-dihydrochalcones are secondary metabolites isolated from many medicinal plants and from the resin known as 'dragon's blood'. Due to their biological potential, our research objective was to determine the possibilities of using biocatalysis processes carried out in deep eutectic solvents (DESs) to obtain 4'-dihydrochalcones as a model compound. The processes were carried out in a culture of the yeast KCh 71 and also in cultures of strains of the genera and . Based on the experiments carried out, an optimum process temperature of 35 °C was chosen, and the most suitable DES contained glycerol as a hydrogen bond donor (HBD). For a medium with 30% water content (DES 11), the conversion observed after 24 h exceeded 70%, while increasing the amount of water to 50% resulted in a similar level of conversion after just 1 h. A fivefold increase in the amount of added substrate resulted in a reduction in conversion, which reached 30.3%. Of the other yeast strains tested, KCh 77 and KCh 4 also proved to be good biocatalysts for the bioreduction process. For these strains, the conversion reached 95.4% and 95.1%, respectively. These findings highlight the potential of yeast as a biocatalyst for the selective reduction of α,β-unsaturated ketones and the possibility of using a DESs as a reaction medium in this process.
4'-二氢查耳酮是从许多药用植物和被称为“龙血”的树脂中分离出来的次生代谢物。由于其具有生物潜力，我们的研究目标是确定使用在深共熔溶剂（DESs）中进行的生物催化过程来获得 4'-二氢查耳酮作为模型化合物的可能性。这些过程是在酵母 KCh 71 的培养物中以及 和 属的菌株的培养物中进行的。根据进行的实验，选择了 35°C 的最佳工艺温度，最适合的 DES 含有甘油作为氢键供体（HBD）。对于水含量为 30%的培养基（DES 11），在 24 小时后观察到的转化率超过 70%，而将水量增加到 50%仅在 1 小时后就达到了类似的转化率。添加的底物量增加五倍会导致转化率降低，达到 30.3%。在所测试的其他酵母菌株中，KCh 77 和 KCh 4 也被证明是生物还原过程的良好生物催化剂。对于这两种菌株，转化率分别达到 95.4%和 95.1%。这些发现强调了酵母作为α，β-不饱和酮选择性还原的生物催化剂的潜力，以及在该过程中使用 DESs 作为反应介质的可能性。
