非常规酵母（NCY）全细胞对α，β-不饱和酮和醛的生物还原作用。

Bioreduction of α,β-unsaturated ketones and aldehydes by non-conventional yeast (NCY) whole-cells.

机构信息

Department of Applied Biology and Industrial Yeasts Collection DBVPG, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.

出版信息

Bioresour Technol. 2011 Mar;102(5):3993-8. doi: 10.1016/j.biortech.2010.12.062. Epub 2010 Dec 22.

DOI:10.1016/j.biortech.2010.12.062

PMID:21232941

Abstract

The bioreduction of α,β-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone) and aldehydes [(S)-(-)-perillaldehyde and α-methyl-cinnamaldehyde] by 23 "non-conventional" yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated CC bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed.

摘要

报告了 23 种“非常规”酵母（NCYs）对α，β-不饱和酮（酮异佛尔酮、2-甲基和 3-甲基环戊烯酮）和醛[(S)-(-)-紫苏醛和α-甲基肉桂醛]的生物还原作用，这些酵母属于 21 个属，包括 Candida、Cryptococcus、Debaryomyces、Hanseniaspora、Kazachstania、Kluyveromyces、Lindnera、Nakaseomyces、Vanderwaltozyma 和 Wickerhamomyces。这些结果突出了 NCYs 作为全细胞生物催化剂用于电子贫乏烯烃选择性生物转化的潜力。少数 NCYs 通过烯酸还原酶催化的共轭 CC 键不对称还原，表现出极高（>90%）甚至完全的酮异佛尔酮和 2-甲基环戊烯酮生物转化产率。从酮类切换到醛类后，催化效率下降。由于低竞争羰基还原酶，有时也观察到高化学选择性。