Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Medical School, University of Pécs, 7624 Pécs, Hungary.
National Laboratory on Human Reproduction, University of Pécs, 7621 Pécs, Hungary.
Int J Mol Sci. 2024 Jul 6;25(13):7423. doi: 10.3390/ijms25137423.
The receptive phase of the uterus is marked by structural and functional maturation of the endometrium. During this limited time span, the blastocyst competency is superimposed on the receptive endometrium. It is a well-known fact that lipid signalling in early-stage pregnancy has a crucial role in successful embryogenesis. In our study, CD-1 mouse uteri after normal and in vitro fertilization (IVF) were investigated at 6.5, 8.5, and 10.5 days of pregnancy. Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry and liquid chromatography coupled tandem mass spectrometry were used for identification of phosphatidylcholine (PC) lipid structures. In the embryonal tissues, PC 32:0 and PC 34:0 were increased, while in the antemesometrial (AM) decidua the two 20:4-containing PCs, PC 36:4 and PC 38:4 were increased. In transferred uterus samples, higher expressions of PC 34:0, PC 34:1, PC 34:2, PC 36:1, and PC 36:2 in mesometrial decidua were seen, whereas the two 20:4-containing PCs, PC 36:4 and PC 38:4 showed increased expression in the AM and lateral decidua. This paper shows a significant spatio-temporal change in lipid metabolism during IVF procedures for the first time.
子宫的接受期以子宫内膜的结构和功能成熟为标志。在这个有限的时间内，囊胚的能力叠加在接受的子宫内膜上。众所周知，脂质信号在早期妊娠中对胚胎成功发育起着至关重要的作用。在我们的研究中，在怀孕 6.5、8.5 和 10.5 天时，研究了正常和体外受精 (IVF) 的 CD-1 小鼠子宫。基质辅助激光解吸/电离飞行时间成像质谱和液相色谱串联质谱用于鉴定磷脂酰胆碱 (PC) 脂质结构。在胚胎组织中，PC 32:0 和 PC 34:0 增加，而在前子宫蜕膜 (AM) 蜕膜中，两种含有 20:4 的 PC，PC 36:4 和 PC 38:4 增加。在转移的子宫样本中，在中隔蜕膜中观察到 PC 34:0、PC 34:1、PC 34:2、PC 36:1 和 PC 36:2 的表达更高，而两种含有 20:4 的 PC，PC 36:4 和 PC 38:4 在 AM 和侧蜕膜中表达增加。本文首次显示了在 IVF 过程中脂质代谢的显著时空变化。
