Pavić Ivan, Kaštelan Nediljko, Adamczyk Arkadiusz, Ivanda Mile
Department for Marine Electrical Engineering and Information Technologies, Faculty of Maritime Studies, Ruđera Boškovića 37, 21000 Split, Croatia.
Faculty of Mechanical and Electrical Engineering, Polish Naval Academy, ul. Smidowicza 69, 81-127 Gdynia, Poland.
Sensors (Basel). 2024 Jul 1;24(13):4284. doi: 10.3390/s24134284.
Raman spectroscopy is a powerful analytical technique based on the inelastic scattering of photons. Conventional macro-Raman spectrometers are suitable for mass analysis but often lack the spatial resolution required to accurately examine microscopic regions of interest. For this reason, the development of micro-Raman spectrometers has been driven forward. However, even with micro-Raman spectrometers, high resolution is required to gain better insight into materials that provide low-intensity Raman signals. Here, we show the development of a micro-Raman spectrometer with implemented zoom lens technology. We found that by replacing a second collimating mirror in the monochromator with a zoom lens, the spectral resolution could be continuously adjusted at different zoom factors, i.e., high resolution was achieved at a higher zoom factor and lower spectral resolution was achieved at a lower zoom factor. A quantitative analysis of a micro-Raman spectrometer was performed and the spectral resolution was analysed by FWHM using the Gaussian fit. Validation was also performed by comparing the results obtained with those of a high-grade laboratory Raman spectrometer. A quantitative analysis was also performed using the ANOVA method and by assessing the signal-to-noise ratio between the two systems.
拉曼光谱是一种基于光子非弹性散射的强大分析技术。传统的宏观拉曼光谱仪适用于大量分析，但往往缺乏准确检测感兴趣微观区域所需的空间分辨率。因此，推动了显微拉曼光谱仪的发展。然而，即使使用显微拉曼光谱仪，也需要高分辨率才能更好地洞察提供低强度拉曼信号的材料。在此，我们展示了一种采用变焦透镜技术的显微拉曼光谱仪的开发。我们发现，通过用变焦透镜替换单色仪中的第二个准直镜，可以在不同的变焦系数下连续调节光谱分辨率，即，在较高的变焦系数下实现高分辨率，在较低的变焦系数下实现较低的光谱分辨率。对显微拉曼光谱仪进行了定量分析，并使用高斯拟合通过半高宽分析光谱分辨率。还通过将获得的结果与高级实验室拉曼光谱仪的结果进行比较来进行验证。还使用方差分析方法并通过评估两个系统之间的信噪比进行了定量分析。
