Vandenabeele Peter, Rousaki Anastasia
Department of Chemistry, Raman Spectroscopy Research Group, Ghent University, S-12, Krijgslaan 281, B-9000 Ghent, Belgium.
Department of Archaeology, Archaeometry Research Group, Ghent University, Sint-Pietersnieuwstraat 35, B-9000 Ghent, Belgium.
Anal Chem. 2021 Nov 23;93(46):15390-15400. doi: 10.1021/acs.analchem.1c03197. Epub 2021 Nov 12.
Raman spectroscopy is a well-appreciated technique in cultural heritage research for its ability to obtain molecular information nondestructively. During Raman mapping experiments, advantage is taken of the excellent spatial resolution of the approach, allowing to visualize the spatial distribution of the molecules. In the current research, macro-Raman mapping is proposed, allowing us to map large areas of an artwork (typically tens or hundreds of square centimeters). Therefore, a new setup is made, using a commercially available mobile Raman spectrometer and fast translation stages. Moreover, the probe is equipped with a triangulator to measure the distance to the surface of the artwork and thus achieving accurate focusing of the Raman probe. Finally, the correct setup is guaranteed by using a calibration module that is designed to allow for spectral calibration and aligning all components of the probe. The use of the method is demonstrated by three cases, where different data processing techniques are illustrated.
拉曼光谱法因其能够无损获取分子信息，在文化遗产研究中是一种备受认可的技术。在拉曼映射实验中，该方法出色的空间分辨率得到了利用，从而能够可视化分子的空间分布。在当前研究中，提出了宏观拉曼映射，使我们能够对一件艺术品的大面积区域（通常为数十或数百平方厘米）进行映射。因此，利用一台商用移动拉曼光谱仪和快速平移台搭建了一个新装置。此外，该探头配备了一个三角测量仪，用于测量到艺术品表面的距离，从而实现拉曼探头的精确聚焦。最后，通过使用一个校准模块确保设置正确，该校准模块旨在进行光谱校准并对准探头的所有部件。通过三个案例展示了该方法的应用，并说明了不同的数据处理技术。
