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Preparative isolation of plasmid DNA with sedimentation field flow fractionation.

作者信息

Schallinger L E, Gray J E, Wagner L W, Knowlton S, Kirkland J J

出版信息

J Chromatogr. 1985 Jul 12;342(1):67-77. doi: 10.1016/s0378-4347(00)84489-8.

DOI:10.1016/s0378-4347(00)84489-8
PMID:3900119
Abstract

Sedimentation field flow fractionation (SFFF) can be used to isolate plasmids preparatively from crude cellular lysates. Total purification time is about 3/4 day, including lysate preparation. The purity and yield of plasmids isolated by SFFF appear to be at least equivalent to those prepared by traditional methods. Molecular-weight data also are supplied rapidly by SFFF without the need for standards.

摘要

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