Septiana Tabita Thertini, Khotijah Lilis, Wiryawan I Komang Gede
Graduate School of Nutrition and Feed Science, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.
Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.
Trop Anim Health Prod. 2024 Jul 13;56(6):210. doi: 10.1007/s11250-024-04045-4.
The study aimed to to evaluate the effect of feeding protected maggot oil at different levels on the ram sperm quality. The study used 15 local rams with an age of approximately 10-12 months and an initial weight of 19.99 ± 3.97 kg. The feeding rate was 4% of body weight per day. Feed was given 3 times a day, specifically in the morning (08.00 WIB), afternoon (12.00 WIB) and evening (16.00 WIB). Water was provided ad libitum. This study used 3 treatments and 5 groups as replicates. The treatments used concentrates with different levels of protected maggot oil: P0(0% protected maggot oil (control)), P1(4% protected maggot oil), and P2(8% protected maggot oil). The variables measured were nutrient consumption, blood cholesterol levels, scrotal circumference, and sperm quality. Blood cholesterol and scrotal circumference measured at the end of the experimental diet. Semen samples were collected and analysed before and at the end of the experimental diet. The data obtained were analysed using ANOVA, with further testing using Duncan's test for significant differences. The results showed that there were no significant differences in the consumption of dry matter, crude protein, crude fiber, scrotal circumference, volume, colour, pH of semen, sperm concentration, live percentage, abnormal percentage, plasma membrane, and acrosome integrity of spermatozoa. There were significantly (p < 0.05) produced higher consumption of oleic and palmitic acids in 8% protected maggot oil compared to 4% treatments, the treatments containing 4% and 8% protected maggot oil produced significantly (p < 0.05) higher consumption of lauric and myristic acids, blood cholesterol levels, and sperm motility than the control. The result indicates that protected maggot oil up to 8% in the ram diet have positive effect on improving the microscopic quality of ram sperm, i.e. increased sperm motility.
本研究旨在评估不同水平的保护性蛆油对公羊精液质量的影响。该研究选用了15只当地公羊，年龄约为10 - 12个月，初始体重为19.99±3.97千克。饲喂量为每天体重的4%。每天分3次投喂饲料，具体为上午（印度尼西亚西部时间08:00）、下午（印度尼西亚西部时间12:00）和晚上（印度尼西亚西部时间16:00）。自由饮水。本研究采用3种处理方法，每组5个重复。处理方法使用了不同水平保护性蛆油的浓缩饲料：P0（0%保护性蛆油（对照））、P1（4%保护性蛆油）和P2（8%保护性蛆油）。测定的变量包括营养物质消耗量、血液胆固醇水平、阴囊周长和精液质量。在实验日粮结束时测量血液胆固醇和阴囊周长。在实验日粮开始前和结束时采集精液样本并进行分析。对获得的数据进行方差分析，进一步使用邓肯检验分析显著差异。结果表明，干物质、粗蛋白、粗纤维的消耗量、阴囊周长、精液体积、颜色、pH值、精子浓度、活率、异常率、精子质膜和顶体完整性方面均无显著差异。与4%处理组相比，8%保护性蛆油组油酸和棕榈酸的消耗量显著更高（p＜0.05），含有4%和8%保护性蛆油的处理组月桂酸和肉豆蔻酸的消耗量、血液胆固醇水平和精子活力均显著高于对照组（p＜0.05）。结果表明，公羊日粮中添加高达8%的保护性蛆油对改善公羊精液微观质量具有积极作用，即提高精子活力。
