Chaves Lima Thyarlon Bergson, Silveira Robson Mateus Freitas, do Rêgo João Paulo Arcelino, de Alencar Araripe Noronha Moura Arlindo, Lobo Carlos Henrique, McManus Concepta, Batista Nielyson Junio Marcos, Pimentel Patrícia Guimarães, das Neves Maria Rosalba Moreira, Landim Aline Vieira
Department of Animal Science, State University of Acaraú Valley (UVA), Sobral, CE, 62.040-370, Brazil.
Department of Animal Science, University of São Paulo (USP), Luiz de Queiroz College of Agriculture, São Paulo, Piracicaba, 13.418-900, Brazil.
Trop Anim Health Prod. 2024 Jul 13;56(6):213. doi: 10.1007/s11250-024-03999-9.
The present study describes the expression of genes in the Longissimus dorsi muscle related to meat quality of hair lambs finished in an Integration Crop-Livestock system. Twenty-eight non-castrated lambs of two breeds, Somalis Brasileira and Santa Inês, at 120 ± 15 days of age, with an average initial live weight of 18 ± 3.1 kg, were kept in a pasture-based finishing system with supplementation. Upon reaching 28 kg body weight, animals were sent for slaughter. Samples of the Longissimus dorsi and Biceps femoris muscle were harvested for analyses of gene expression and physicochemical properties. Significant differences were detected between the breeds for tissue and chemical composition, whereas the physical aspects did not differ. We observed the expression of six genes related to lipid synthesis (acetyl-CoA carboxylase [ACACA], fatty acid synthase [FAS], stearoyl-CoA desaturase [SCD], lipoprotein lipase [LPL], cell death-inducing DFFA-like effector A [CIDEA], and thyroid hormone responsive [THRSP]) and six genes related to molecular synthesis (myostatin [MSTN], growth differentiation factor 8 [GDF8], insulin-like growth factor 1 [IGF1], insulin-like growth factor 2 [IGF2], delta-like 1 homolog [DLK1], and growth hormone receptor [GHr]) in both breeds. The Santa Inês breed and the Somalis Brasileira showed similar expression patterns of genes related to lipogenesis and myogenesis of the Longissimus dorsi muscle, with the exception of the THRSP gene, in which the Somalis Brasileira have more receptors for the action of thyroid hormones, which resulted in greater thickness of fat in the carcass (subcutaneous fat) and higher lipid content in the chemical composition of the meat.
本研究描述了在农牧结合系统中育肥的毛用羔羊背最长肌中与肉质相关的基因表达情况。选取28只未去势的索马里巴西兰和圣伊内斯两个品种的羔羊，年龄为120±15天，平均初始体重为18±3.1千克，将其饲养在以牧场为基础并进行补饲的育肥系统中。体重达到28千克后，将动物送去屠宰。采集背最长肌和股二头肌样本，用于基因表达分析和理化性质分析。在组织和化学成分方面，两个品种之间检测到显著差异，而物理方面没有差异。我们观察到两个品种中与脂质合成相关的六个基因（乙酰辅酶A羧化酶[ACACA]、脂肪酸合酶[FAS]、硬脂酰辅酶A去饱和酶[SCD]、脂蛋白脂肪酶[LPL]、细胞死亡诱导DFFA样效应因子A[CIDEA]和甲状腺激素反应蛋白[THRSP]）以及与分子合成相关的六个基因（肌肉生长抑制素[MSTN]、生长分化因子8[GDF8]、胰岛素样生长因子1[IGF1]、胰岛素样生长因子2[IGF2]、类Delta样蛋白1[DLK1]和生长激素受体[GHr]）的表达情况。圣伊内斯品种和索马里巴西兰品种在背最长肌的脂肪生成和肌生成相关基因的表达模式上相似，但THRSP基因除外，在该基因方面，索马里巴西兰品种具有更多甲状腺激素作用的受体，这导致胴体脂肪厚度更大（皮下脂肪）以及肉的化学成分中脂质含量更高。
