Su R, Sun W, Li D, Wang Q Z, Lv X Y, Musa H H, Chen L, Zhang Y F, Wu W Z
Animal Science and Technology College, Yangzhou University, Yangzhou, China
Animal Science and Technology College, Yangzhou University, Yangzhou, China.
Genet Mol Res. 2014 Dec 4;13(4):10308-19. doi: 10.4238/2014.December.4.26.
The aim of the present study was to detect delta-like 1 ho-molog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality. The diameter and density of muscle fibers and tenderness of the longissimus dorsi were measured. Growth stage, but not sex, significantly affected DLK1 and IGF-I expression. DLK1 and IGF-I expression in the sheep longissimus dorsi gradually increased with growth, but also decreased during some periods. These results suggest that different growth stages significantly affect DLK1 and IGF-I gene expression in sheep muscle tissue. The ex-pression of DLK1 and IGF-I genes were positively and significantly (P < 0.01) correlated with muscle fiber diameter and muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. Muscle fiber diameter was positively and significantly (P < 0.01) correlated with muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. In addition, DLK-1 expression was significantly (P < 0.01) and positively correlated with IGF-I expression.
本研究旨在检测不同生长阶段湖羊背最长肌中类Delta样蛋白1（DLK1）和胰岛素样生长因子-I（IGF-I）基因的表达，并研究这些基因与肉质之间的关联。测定了背最长肌的肌纤维直径、密度和嫩度。生长阶段而非性别对DLK1和IGF-I的表达有显著影响。湖羊背最长肌中DLK1和IGF-I的表达随生长而逐渐增加，但在某些时期也会下降。这些结果表明，不同生长阶段对绵羊肌肉组织中DLK1和IGF-I基因的表达有显著影响。DLK1和IGF-I基因的表达与肌纤维直径和肌纤维剪切力呈显著正相关（P<0.01），与肌纤维密度呈显著负相关（P<0.01）。肌纤维直径与肌纤维剪切力呈显著正相关（P<0.01），与肌纤维密度呈显著负相关（P<0.01）。此外，DLK-1的表达与IGF-I的表达呈显著正相关（P<0.01）。
