Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China.
Talanta. 2024 Oct 1;278:126531. doi: 10.1016/j.talanta.2024.126531. Epub 2024 Jul 10.
Herein, the surface-enhanced Raman scattering (SERS) platform was combined with an azo coupling reaction and an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes for the detection of histamine. The praseodymium oxide on aluminum alloy was successfully synthesized by the rare-earth-salt-solution boiling bath method and modified by stearic acid. Its surface exhibits a water contact angle (WCA) of 125.0°. Through the azo derivatization reaction with 3-amino-5-mercapto-1,2,4-triazole (AMTA) diazonium salts, histamine can be converted into the derivatization product with higher Raman activity. The mixture of the derivatization product and β-cyclodextrin-modified Ag nanoparticles (β-CD-AgNPs) were dropped onto the surface of an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes, and dried for SERS measurement. The intensity ratio between the SERS peaks at 1246 cm and 1104 cm (I/I) of the derivatization product was used for the quantification of histamine. Under the selected conditions, the limit of detection (LOD) and the limit of quantification (LOQ) for this method were 7.2 nM (S/N = 3) and 24 nM (S/N = 10), respectively. The relative standard deviation (RSD) of this method for the determination of 1 μM histamine was 6.1 % (n = 20). The method was also successfully used for the determination of histamine in fish samples with recoveries ranging from 92 % to 111 %. The present method is simple, sensitive, reliable, and may provide a new approach for preparing the composite hydrophobic layer that can enhance SERS signals through hydrophobic condensation effect. Meanwhile, it may have a promising future in the determination of small molecular compounds containing an imidazole ring.
本文结合表面增强拉曼散射（SERS）平台和偶氮耦合反应，以及用镨氧化物和硬脂酸复合物覆盖疏水层的铝合金，用于组胺的检测。铝合金上的镨氧化物是通过稀土盐溶液沸腾浴法成功合成的，并通过硬脂酸进行了修饰。其表面的水接触角（WCA）为 125.0°。通过与 3-氨基-5-巯基-1,2,4-三唑（AMTA）重氮盐的偶氮衍生化反应，组胺可以转化为具有更高拉曼活性的衍生化产物。将衍生化产物与β-环糊精修饰的银纳米粒子（β-CD-AgNPs）的混合物滴加到覆盖有镨氧化物和硬脂酸复合物的疏水层的铝合金表面上，并干燥进行 SERS 测量。衍生化产物的 SERS 峰在 1246 cm 和 1104 cm 处的强度比（I/I）用于组胺的定量。在选定的条件下，该方法的检测限（LOD）和定量限（LOQ）分别为 7.2 nM（S/N = 3）和 24 nM（S/N = 10）。该方法用于测定 1 μM 组胺的相对标准偏差（RSD）为 6.1%（n = 20）。该方法还成功地用于鱼类样品中组胺的测定，回收率在 92%至 111%之间。本方法简单、灵敏、可靠，可为通过疏水缩合效应增强 SERS 信号的复合疏水层的制备提供新途径。同时，它在含有咪唑环的小分子化合物的测定中可能具有广阔的前景。
