Xie Zhengjun, Wang Yang, Chen Yisheng, Xu Xueming, Jin Zhengyu, Ding Yunlian, Yang Na, Wu Fengfeng
The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China.
Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China.
Food Chem. 2017 Sep 1;230:547-552. doi: 10.1016/j.foodchem.2017.03.081. Epub 2017 Mar 15.
Reliable screening of histamine in fish was of urgent importance for food safety. This work presented a highly selective surface enhanced Raman spectroscopy (SERS) method mediated by thin-layer chromatography (TLC), which was tailored for identification and quantitation of histamine. Following separation and derivatization with fluram, plates were assayed with SERS, jointly using silver nanoparticle and NaCl. The latter dramatically suppressed the masking effect caused by excessive fluram throughout the plate, thus offering clear baseline and intensive Raman fingerprints specific to the analyte. Under optimized conditions, the usability of this method was validated by identifying the structural fingerprints of both targeted and unknown compounds in fish samples. Meanwhile, the quantitative results of this method agreed with those by an HPLC method officially suggested by EU for histamine determination. Showing remarkable cost-efficiency and user-friendliness, this facile TLC-SERS method was indeed screening-oriented and may be more attractive to controlling laboratories of limited resource.
可靠地筛查鱼类中的组胺对食品安全至关重要。这项工作提出了一种由薄层色谱法（TLC）介导的高选择性表面增强拉曼光谱法（SERS），该方法专为组胺的鉴定和定量而定制。在用氟胺进行分离和衍生化后，用SERS对板进行检测，联合使用银纳米颗粒和氯化钠。后者极大地抑制了整个板上过量氟胺引起的掩蔽效应，从而提供了清晰的基线和特定于分析物的强烈拉曼指纹。在优化条件下，通过识别鱼样品中目标化合物和未知化合物的结构指纹，验证了该方法的可用性。同时，该方法的定量结果与欧盟官方建议的用于组胺测定的高效液相色谱法的结果一致。这种简便的TLC-SERS方法具有显著的成本效益和用户友好性，确实以筛查为导向，可能对资源有限的控制实验室更具吸引力。
