Jin Chengcheng, Yang Shuang, Zheng Junlei, Chai Fang, Tian Miaomiao
Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025, PR China.
Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025, PR China.
Food Chem. 2024 Nov 30;459:140369. doi: 10.1016/j.foodchem.2024.140369. Epub 2024 Jul 10.
The improper use of organophosphate pesticides (OPs) can lead to residue posing a serious threat to human health and environment. Therefore, the development of a simple, portable, and sensitive detection method is crucial. Herein, a bioenzyme-nanozyme-chromogen all-in-one paper-based sensor was synthesized. Initially, the Ce/Zr-MOF with peroxidase-like activity was grown on filter paper (FP) using in-situ solvent thermal method, resulting in Ce/Zr-MOF@FP. Subsequently, the AChE-ChO-TMB system was immobilized onto Ce/Zr-MOF@FP using biocompatible gelatin, which enhanced cascade catalysis efficiency through the proximity effect. Based on the inhibition principle of OPs on AChE, we integrated this sensor with Python-based image recognition algorithm to achieve detection of OPs. Using 2,2-dichlorovinyl dimethyl phosphate (DDVP) as a model of OPs, it has good detection performance with a detection limit of 0.32 ng mL and a recovery rate range of 95-107%. The potential for on-site detection of DDVP residues in vegetables and fruit samples is highly promising.
有机磷酸酯类农药（OPs）的不当使用会导致残留，对人类健康和环境构成严重威胁。因此，开发一种简单、便携且灵敏的检测方法至关重要。在此，合成了一种生物酶-纳米酶-显色剂一体化的纸质传感器。首先，采用原位溶剂热法在滤纸（FP）上生长具有类过氧化物酶活性的Ce/Zr-MOF，得到Ce/Zr-MOF@FP。随后，使用生物相容性明胶将AChE-ChO-TMB体系固定在Ce/Zr-MOF@FP上，通过邻近效应提高级联催化效率。基于OPs对AChE的抑制原理，将该传感器与基于Python的图像识别算法相结合，实现对OPs的检测。以2,2-二氯乙烯基二甲基磷酸酯（DDVP）作为OPs的模型，该传感器具有良好的检测性能，检测限为0.32 ng/mL，回收率范围为95-107%。在蔬菜和水果样品中现场检测DDVP残留具有很高的潜力。
